CS Citrate Synthase Antibody (JU80-30)

Western Blot: CS Citrate Synthase Antibody (JU80-30) [NBP2-75450] - Analysis of Citrate synthetase on Hela cell using anti-Citrate synthetase antibody at 1/1,000 dilution.Positive control:Lane 1: Rat kidney tissue Lane 2: HelaLane 3: Mouse brain tissue

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (JU80-30) [NBP2-75450] - Analysis of paraffin-embedded rat epididymis tissue using anti-Citrate synthetase antibody. Counter stained with hematoxylin.

Flow Cytometry: CS Citrate Synthase Antibody (JU80-30) [NBP2-75450] - Analysis of 293T cells with Citrate synthetase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (JU80-30) [NBP2-75450] - Analysis of paraffin-embedded human tonsil tissue using anti-Citrate synthetase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (JU80-30) [NBP2-75450] - Analysis of paraffin-embedded human kidney tissue using anti-Citrate synthetase antibody. Counter stained with hematoxylin.

Product Discontinued

CS Citrate Synthase Antibody (JU80-30) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human CS Citrate Synthase aa 417-466 / 466. (SwissProt: O75390 Human; SwissProt: Q9CZU6 Mouse; SwissProt: Q8VHF5 Rat)
• Localization: Mitochondrion matrix.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CS Citrate Synthase Antibody (JU80-30)

• citrate (Si)-synthase
• citrate synthase
• citrate synthase, mitochondrial
• CS Citrate Synthase
• EC 2.3.3
• EC 2.3.3.1

Background

The protein encoded by the CS Citrate Synthase gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citratefrom oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. Thisprotein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. (provided byRefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CS