CS Citrate Synthase Antibody (CL2545) - Azide and BSA Free

Staining in HeLa cell line with Anti-CS Citrate Synthase monoclonal antibody) .

Staining in MCF7 cell line with Anti-CS Citrate Synthase monoclonal antibody) .

Staining in U2OS cell line with Anti-CS Citrate Synthase monoclonal antibody) .

Lane 1: Marker [kDa]Lane 2:Human cell line U-251 MG

Staining in U251 cell line with Anti-CS Citrate Synthase monoclonal antibody) .

Analysis in human heart muscle and liver tissues using NBP3-44449 antibody. Corresponding CS Citrate Synthase RNA-seq data are presented for the same tissues.

Staining in A431 cell line with Anti-CS Citrate Synthase monoclonal antibody) .

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.

Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL2545
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

CS Citrate Synthase Antibody (CL2545) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of O75390, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for CS Citrate Synthase Antibody (CL2545) - Azide and BSA Free

• citrate (Si)-synthase
• citrate synthase
• citrate synthase, mitochondrial
• CS Citrate Synthase
• EC 2.3.3
• EC 2.3.3.1

Background

The protein encoded by the CS Citrate Synthase gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citratefrom oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. Thisprotein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. (provided byRefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CS