CRM1 Antibody

Western Blot: CRM1 Antibody [NB100-56493] - Analysis of Exportin-1 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 1.5 ug/ml.

Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-56493] - Analysis of human spleen using this antibody at 10 ug/ml.

Product Discontinued

CRM1 Antibody Summary

• Immunogen: Amino acids 390-408 (ASPLLSGSQHFDVPPRRQL) of human Exportin-1 protein were used as the immunogen (NP_003391).
• Predicted Species: Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XPO1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-3 ug/ml
• Theoretical MW: 123 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Jurkat Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for CRM1 Antibody

• Chromosome region maintenance 1 protein homolog
• DKFZp686B1823
• emb
• exp1
• exportin 1 (CRM1 homolog, yeast)
• exportin 1 (CRM1, yeast, homolog)
• Exportin-1 (required for chromosome region maintenance)
• exportin-1
• yeast, homolog

Background

Nuclear transport receptors of karyopherin/importin-beta superfamily mediate various transport pathways between nucleus and cytoplasm. Exportin-1, a human homolog of yeast CRM1, is a novel karyopherin export receptor that mediates leucine-rich NES-dependent protein export. It interacts with RanGTP through its Ran-binding domain and also with nucleoporins including Nup214, Nup50, Nup42 and Nup159 during the process of exporting NES-containing proteins. It mediates the export of many cellular and viral proteins and ribonucleoproteins including HIV Rev Protein, ERK3, PKI, IRF-5, Cyclin-B, MAPKK, MAPKAP kinase 2, Snurportin, Cap-binding protein, and certain U snRNAs. Reports suggest exportin-1 also has a role in the regulation of NFAT and AP-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRM1 Antibody (NB100-56493)(1)

Publication using NB100-56493

• Bagheri H, Badduke C, Qiao Y et al. Identifying candidate genes for 2p15p16.1 microdeletion syndrome using clinical, genomic, and functional analysis. JCI Insight. 2016-03-17 [PMID: 27699255] (WB, Human)

Bioinformatics

• Gene Symbol: XPO1
• Entrez:
  • 7514(Human)
  • 103573(Mouse)
  • 85252(Rat)
• Uniprot:
  • O14980(Human)