CRM1 Antibody (JB35-22)

Western Blot: CRM1 Antibody (JB35-22) [NBP2-75448] - Analysis of CRM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293T cell lysate Predicted band size: 123 kDa Observed band size: 110 kDa

Immunocytochemistry/Immunofluorescence: CRM1 Antibody (JB35-22) [NBP2-75448] - Staining CRM1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CRM1 Antibody (JB35-22) [NBP2-75448] - Analysis of paraffin-embedded human lymph node tissue with Rabbit anti-CRM1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: CRM1 Antibody (JB35-22) [NBP2-75448] - Analysis of K562 cells with CRM1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: CRM1 Antibody (JB35-22) [NBP2-75448] - Analysis of CRM1 on 293T and Hela cell lysates using anti-CRM1 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: CRM1 Antibody (JB35-22) [NBP2-75448] - Staining CRM1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CRM1 Antibody (JB35-22) [NBP2-75448] - Staining CRM1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CRM1 Antibody (JB35-22) [NBP2-75448] - Analysis of paraffin-embedded human tonsil tissue using anti-CRM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CRM1 Antibody (JB35-22) [NBP2-75448] - Analysis of paraffin-embedded human lung cancer tissue using anti-CRM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CRM1 Antibody (JB35-22) [NBP2-75448] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-CRM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CRM1 Antibody (JB35-22) [NBP2-75448] - Analysis of paraffin-embedded rat skeletal muscle tissue using anti-CRM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CRM1 Antibody (JB35-22) [NBP2-75448] - Analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-CRM1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: CRM1 Antibody (JB35-22) [NBP2-75448] - Analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-CRM1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

CRM1 Antibody (JB35-22) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within C-terminal Human CRM1. (SwissProt: O14980 Human; SwissProt: Q6P5F9 Mouse; SwissProt: Q80U96 Rat)
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: XPO1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:400
  • Western Blot 1:500
• Theoretical MW: 123 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CRM1 Antibody (JB35-22)

• Chromosome region maintenance 1 protein homolog
• DKFZp686B1823
• emb
• exp1
• exportin 1 (CRM1 homolog, yeast)
• exportin 1 (CRM1, yeast, homolog)
• Exportin-1 (required for chromosome region maintenance)
• exportin-1
• yeast, homolog

Background

Official Gene Symbol: XPO1 Gen Bank Accession Number: NP_003391 Gene ID: 7514 (human) Gene Map Locus: 2p16 (human) Nuclear transport receptors of karyopherin/importin-beta superfamily mediate various transport pathways between nucleus and cytoplasm. Exportin-1, a human homolog of yeast CRM1, is a novel karyopherin export receptor that mediates leucine-rich NES-dependent protein export. It interacts with RanGTP through its Ran-binding domain and also with nucleoporins including Nup214, Nup50, Nup42 and Nup159 during the process of exporting NES-containing proteins. It mediates the export of many cellular and viral proteins and ribonucleoproteins including HIV Rev Protein, ERK3, PKI, IRF-5, Cyclin-B, MAPKK, MAPKAP kinase 2, Snurportin, Cap-binding protein, and certain U snRNAs. Reports suggest exportin-1 also has a role in the regulation of NFAT and AP-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: XPO1