CRHR2/CRF2 Antibody - BSA Free

Western Blot: CRHR2/CRF2 Antibody [NB100-56485] - Analysis of CRHR2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Both of the isoforms detected by this antibody.

Simple Western: CRHR2/CRF2 Antibody [NB100-56485] - Shows a specific band for Corticotropin Releasing Factor 2 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Ma, Pm
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CRHR2/CRF2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CRHR2/CRF2 Antibody - BSA Free (NB100-56485) is a polyclonal antibody validated for use in WB, ICC/IF and Simple Western. Anti-CRHR2/CRF2 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 75-125 of human CRHR2 was used as the immunogen.
• Specificity: There are three isoforms of CRHR2 in human: alpha is 411 amino acids, beta is 438 amino acids, and gamma is 397 amino acids.
• Predicted Species: Primate (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRHR2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Simple Western 1:200
  • Western Blot 0.5-2 ug/ml
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 49 kDa
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID:33344730) 94% sequence identity with Rhesus monkey

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CRHR2/CRF2 Antibody - BSA Free

• corticotropin releasing hormone receptor 2
• corticotropin-releasing factor receptor 2
• Corticotropin-releasing hormone receptor 2
• CRF2
• CRF2R
• CRFR2
• CRF-R2
• CRF-R-2
• CRFR-2
• CRF-RB
• CRH2R
• CRHR2
• CRH-R2
• CRH-R-2
• HM-CRF

Background

CRHR2 is a novel member of Secretin-family-GPCR proteins that functions as a functional receptor for CRF. It is expressed as 3 isoforms; alpha, beta and gamma. These isoforms primarily differ in their N-terminal sequence and tissue distribution. Apart from CRF, other ligands of CRHR2 include UcnI, UcnII and UcnIII. CRHR2 is involved in stress responses, cardiovascular function and gastric motility. Studies on CRHR2 deficient mice suggested a central anxiolytic effect of CRHR2. Northern Blot analysis detects CRHR2 expression in brain, hypothalamus, heart, GI, lung, skeletal muscle and vasculature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRHR2/CRF2 Antibody (NB100-56485)(5)

Publications using NB100-56485

• Lee, JS;Kang, JY;Park, SY;Hwang, SJ;Bae, SJ;Son, CG; Central 5-HTergic hyperactivity induces myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS)-like pathophysiology Journal of translational medicine 2024-01-08 [PMID: 38191373]
• M D, Cs M, Gn P et al. Social isolation alters behavior, the gut-immune-brain axis, and neurochemical circuits in male and female prairie voles Neurobiology of Stress 2020-11-01 [PMID: 33344730] (WB, Mammal)
  
  Details:
  Prairie vole brain proteins were analyzed by Western blot analysis.
• D'Costa S, Ayyadurai S, Gibson AJ et al. Mast Cell CRF2 Suppresses Mast Cell Degranulation and Limits the Severity of Anaphylaxis and Stress-Induced Intestinal Permeability. J. Allergy Clin. Immunol. 2018-11-12 [PMID: 30439403] (ICC/IF, Mouse)
• Roh J, Shin SJ, Lee AN et al. RGS1 expression is associated with poor prognosis in multiple myeloma. J Clin Pathol 2016-07-21 [PMID: 27445341] (WB, Mouse)
• Huang M, Kempuraj D, Papadopoulou N et al. Urocortin induces interleukin-6 release from rat cardiomyocytes through p38 MAP kinase, ERK and NF-kappaB activation. J Mol Endocrinol. 2009-05-01 [PMID: 19211730] (WB, Rat)
  
  Details:
  WB (rat cardiomyocytes, cortex and cerebellum), Fig. 2c.

Review for CRHR2/CRF2 Antibody (NB100-56485) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer WB Other 09/11/2014

Summary

• Application: Western Blot
• Sample Tested: brain lysate
• Species: Other

Bioinformatics

• Gene Symbol: CRHR2
• Entrez:
  • 1395(Human)
  • 12922(Mouse)
  • 64680(Rat)
• Uniprot:
  • NP_001874(Human)