CRHR2/CRF2 Antibody

Images

 
Western Blot: CRHR2/CRF2 Antibody [NB100-56485] - Analysis of CRHR2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Both of the isoforms detected by this antibody.
Simple Western: CRHR2/CRF2 Antibody [NB100-56485] - Shows a specific band for Corticotropin Releasing Factor 2 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CRHR2/CRF2 Antibody Summary

Immunogen
A portion of amino acids 75-125 of human CRHR2 was used as the immunogen.
Specificity
There are three isoforms of CRHR2 in human: alpha is 411 amino acids, beta is 438 amino acids, and gamma is 397 amino acids.
Predicted Species
Primate (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CRHR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Simple Western 1:200
  • Immunocytochemistry/Immunofluorescence
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30439403).
Control
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 1 Review rated 5
using
NB100-56485 in the following applications:

Publications
Read Publications using
NB100-56485 in the following applications:

Reactivity Notes

94% sequence identity with Rhesus monkey

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CRHR2/CRF2 Antibody

  • corticotropin releasing hormone receptor 2
  • corticotropin-releasing factor receptor 2
  • Corticotropin-releasing hormone receptor 2
  • CRF2
  • CRF2R
  • CRFR2
  • CRF-R2
  • CRF-R-2
  • CRFR-2
  • CRF-RB
  • CRH2R
  • CRHR2
  • CRH-R2
  • CRH-R-2
  • HM-CRF

Background

CRHR2 is a novel member of Secretin-family-GPCR proteins that functions as a functional receptor for CRF. It is expressed as 3 isoforms; alpha, beta and gamma. These isoforms primarily differ in their N-terminal sequence and tissue distribution. Apart from CRF, other ligands of CRHR2 include UcnI, UcnII and UcnIII. CRHR2 is involved in stress responses, cardiovascular function and gastric motility. Studies on CRHR2 deficient mice suggested a central anxiolytic effect of CRHR2. Northern Blot analysis detects CRHR2 expression in brain, hypothalamus, heart, GI, lung, skeletal muscle and vasculature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CRHR2/CRF2 Antibody (NB100-56485)(3)

Review for CRHR2/CRF2 Antibody (NB100-56485) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB100-56485:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot CRHR2/CRF2 NB100-56485
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reviewed by:
Anonymous
WB Other 09/11/2014
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Summary

ApplicationWestern Blot
Sample Testedbrain lysate
SpeciesOther

Blocking

Blocking Details5% milk in TTBS

Primary Anitbody

Dilution Ratio1:500, 2nights, 4C, 5% milk in TTBS

Secondary Antibody

Secondary DescriptionIRDye680LT goat anti-rabbit
Secondary Manufacturer Cat#Li-Cor 926-68021
Secondary Concentration1:20K

Details

Detection NotesOdyssay Scanner

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CRHR2/CRF2 Antibody (NB100-56485) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for CRHR2/CRF2 Antibody (NB100-56485)

Discover related pathways, diseases and genes to CRHR2/CRF2 Antibody (NB100-56485). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CRHR2/CRF2 Antibody (NB100-56485)

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PTMs for CRHR2/CRF2 Antibody (NB100-56485)

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Research Areas for CRHR2/CRF2 Antibody (NB100-56485)

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Recent Reviews

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1
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Anonymous
09/11/2014
Application: WB
Species: Other

Bioinformatics

Gene Symbol CRHR2
Entrez
Uniprot
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