CRHR1/CRF1 Antibody

Western Blot: CRHR1/CRF1 Antibody [NBP1-00175] - Staining of Human Colon (A), Cerebellum (B), Rat Brain (C) and Mouse spinal Cord (D) lysates (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: CRHR1/CRF1 Antibody [NBP1-00175] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunocytochemistry/Immunofluorescence: CRHR1/CRF1 Antibody [NBP1-00175] - Immunofluorescence analysis of paraformaldehyde fixed Neuro2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane, and vesicle staining. The nuclear stain is DAPI (blue).Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CRHR1/CRF1 Antibody Summary

• Description: Novus Biologicals Goat CRHR1/CRF1 Antibody (NBP1-00175) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and Simple Western. Anti-CRHR1/CRF1 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NEEKKSKVHYH corresponding to internal region according to NP_004373.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CRHR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Peptide ELISA Detection limit 1:128000
  • Simple Western 1:100
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: Western blot: Approx 60kDa band observed in Human Brain (Cerebellum), Colon, Rat Brain and Mouse spinal cord lysates (calculated MW of 47.7kDa according to NP_004373.2). Primary incubation was 1 hour. IHC reported in scientific literature (PMID: 22570383). Use in ICC/IF reported in scientific literature (PMID: 30439403).
  See Simple Western Antibody Database for Simple Western validation: Tested in Brain, separated by Size, antibody dilution of 1:100

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CRHR1/CRF1 Antibody

• CHR-R1
• corticotropin releasing hormone receptor 1
• corticotropin-releasing factor receptor 1
• corticotropin-releasing factor type 1 receptor
• Corticotropin-releasing hormone receptor 1
• CRF1
• CRFR
• CRF-R
• CRFR1
• CRF-R1
• CRF-R-1
• CRFR-1
• CRHR1
• CRH-R-1
• CRHR1f
• CRH-R1h
• CRHR1L
• CRHRCRH-R1
• seven transmembrane helix receptor

Background

The corticotropin-releasing hormone receptor binds to corticotropin-releasing hormone (MIM 122560), a potent mediator of endocrine, autonomic, behavioral, and immune responses to stress.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRHR1/CRF1 Antibody (NBP1-00175)(6)

Publications using NBP1-00175

• Ritchie JL, Walters JL, Galliou JMC Et al. Basolateral amygdala corticotropin-releasing factor receptor type 1 regulates context-cocaine memory strength during reconsolidation in a sex-dependent manner Neuropharmacology 2021-12-01 [PMID: 34610289] (Simple Western, Rat)
• D'Costa S, Ayyadurai S, Gibson AJ et al. Mast Cell CRF2 Suppresses Mast Cell Degranulation and Limits the Severity of Anaphylaxis and Stress-Induced Intestinal Permeability. J. Allergy Clin. Immunol. 2018-11-12 [PMID: 30439403] (ICC/IF, Mouse)
• Wang S, Wang C, Yu Z et al. Agarwood Essential Oil Ameliorates Restrain Stress-Induced Anxiety and Depression by Inhibiting HPA Axis Hyperactivity. Int J Mol Sci. 2018-11-05 [PMID: 30400578] (WB, Mouse)
• Jungling K, Liu X, Lesting J et al. Activation of neuropeptide S expressing neurons in the locus coeruleus by corticotropin-releasing factor. J Physiol 2012-05-08 [PMID: 22570383] (IHC-P, Mouse)
• Rissman RA, Lee KF, Vale W, Sawchenko PE. Corticotropin-releasing factor receptors differentially regulate stress-induced tau phosphorylation. J Neurosci 2007-06-13 [PMID: 17567816]
• Xu G, Rabadan-Diehl C, Nikodemova M et al. Inhibition of corticotropin releasing hormone type-1 receptor translation by an upstream AUG triplet in the 5' untranslated region. Mol Pharmacol 2001-03-01 [PMID: 11179443]

Bioinformatics

• Gene Symbol: CRHR1
• Entrez:
  • 1394(Human)
  • 12921(Mouse)
  • 58959(Rat)
• Uniprot:
  • P34998(Human)