Western Blot: CRHR1/CRF1 Antibody [NBP1-00175] - Staining of Human Colon (A), Cerebellum (B), Rat Brain (C) and Mouse spinal Cord (D) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
Western blot: Approx 60kDa band observed in Human Brain (Cerebellum), Colon, Rat Brain and Mouse spinal cord lysates (calculated MW of 47.7kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol. 2001 Mar;59(3):485-92; PMID: 11179443). Primary incubation was 1 hour. IHC reported in (PMID: 22570383). Use in ICC/IF reported in scientific literature (PMID: 30439403).
Read Publications using NBP1-00175 in the following applications:
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for CRHR1/CRF1 Antibody
corticotropin releasing hormone receptor 1
corticotropin-releasing factor receptor 1
corticotropin-releasing factor type 1 receptor
Corticotropin-releasing hormone receptor 1
seven transmembrane helix receptor
The corticotropin-releasing hormone receptor binds to corticotropin-releasing hormone (MIM 122560), a potent mediator of endocrine, autonomic, behavioral, and immune responses to stress.[supplied by OMIM]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.