CRHR1/CRF1 Antibody

Images

 
Western Blot: CRHR1/CRF1 Antibody [NBP1-00175] - Staining of Human Colon (A), Cerebellum (B), Rat Brain (C) and Mouse spinal Cord (D) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: CRHR1/CRF1 Antibody [NBP1-00175] - NBP1-00175 (0.3 ug/ml) staining in green a dendrite of a neuronal cell in C57 mouse cortex. Counter-stained with DAPI (in blue). Data kindly ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CRHR1/CRF1 Antibody Summary

Immunogen
Peptide with sequence C-NEEKKSKVHYH corresponding to internal region according to NP_004373.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CRHR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunocytochemistry/Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:128000
Application Notes
Western blot: Approx 60kDa band observed in Human Brain (Cerebellum), Colon, Rat Brain and Mouse spinal cord lysates (calculated MW of 47.7kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol. 2001 Mar;59(3):485-92; PMID: 11179443).
IHC: This product was successfully used on cryosection of mouse brain (PMID: 22570383)
Immunofluorescence: This product was successfully used in immunofluorescence on mouse brain at 2ug/ml when incubated at 4C for 40h.
Publications
Read Publications using
NBP1-00175 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CRHR1/CRF1 Antibody

  • CHR-R1
  • corticotropin releasing hormone receptor 1
  • corticotropin-releasing factor receptor 1
  • corticotropin-releasing factor type 1 receptor
  • Corticotropin-releasing hormone receptor 1
  • CRF1
  • CRFR
  • CRF-R
  • CRFR1
  • CRF-R1
  • CRF-R-1
  • CRFR-1
  • CRHR1
  • CRH-R-1
  • CRHR1f
  • CRH-R1h
  • CRHR1L
  • CRHRCRH-R1
  • seven transmembrane helix receptor

Background

The corticotropin-releasing hormone receptor binds to corticotropin-releasing hormone (MIM 122560), a potent mediator of endocrine, autonomic, behavioral, and immune responses to stress.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CRHR1/CRF1 Antibody (NBP1-00175)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for CRHR1/CRF1 Antibody (NBP1-00175)

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Bioinformatics

Gene Symbol CRHR1
Entrez
Uniprot