CPNE3 Antibody

Western Blot: CPNE3 Antibody [NBP1-85939] - Analysis in human cell line SK-BR-3.

Immunocytochemistry/Immunofluorescence: CPNE3 Antibody [NBP1-85939] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: CPNE3 Antibody [NBP1-85939] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CPNE3 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MAAQCVTKVALNVSCANLLDKDIGSKSDPLCVLFLNTSGQQWYEVERTERIKNCLNPQFSKTFIIDYYFEVVQKLKFGVYDIDNK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CPNE3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CPNE3 Protein (NBP1-85939PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%), Mouse (87%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CPNE3 Antibody

• copine IIICPN3KIAA0636
• copine-3
• PRO1071

Background

Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene encodes a protein which contains two type II C2 domains in the amino-terminus and an A domain-like sequence in the carboxy-terminus. The A domain mediates interactions between integrins and extracellular ligands. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CPNE3 Antibody (NBP1-85939)(1)

Publication using NBP1-85939

• Talaat IM, Abu-Gharbieh E, Hussein A et al. Prognostic Value of Copine 1 in Patients With Renal Cell Carcinoma Anticancer research 2022-01-01 [PMID: 34969745]

Bioinformatics

• Gene Symbol: CPNE3