CORO1B Antibody

Images

 
Western Blot: CORO1B Antibody [NB100-61040] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-61040 used for WB at 0.04 ug/ml ...read more
Immunohistochemistry-Paraffin: CORO1B Antibody [NB100-61040] - Human colon carcinoma. Antibody used at a dilution of 1:1,000.
Immunohistochemistry-Paraffin: CORO1B Antibody [NB100-61040] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-Coronin 2 used at a dilution of 1:250.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

CORO1B Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 450 and 489 of human Coronin, actin binding protein, 1B using the numbering given in entry NP_065174.1 (GeneID 57175).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CORO1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
CORO1B Lysate (NBL1-09396)
Publications
Read Publication using NB100-61040.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CORO1B Antibody

  • coronin, actin binding protein, 1B
  • coronin, actin-binding protein, 1B
  • coronin-1B
  • coronin-2
  • DKFZp762I166

Background

Coronin 2 is an F-actin binding protein that plays an important role in processes involving actin dynamics such as fibroblast migration, and phagocytosis. Coronin 2 influences actin dynamics by promoting actin depolymerization. It has been shown to interact with the Arp2/3 complex and inhibit Arp2/3-induced actin nucleation. Additionally, it can regulate motility by regulating the actin severing and disassembling activity of cofilin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CORO1B Antibody (NB100-61040)(1)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CORO1B
Entrez
Uniprot