CORO1B Antibody

Western Blot: CORO1B Antibody [NB100-61040] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-61040 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Coronin 2 was also immunoprecipitated by rabbit anti-Coronin 2 antibody NB100-61039, which recognizes an upstream epitope.

Immunohistochemistry-Paraffin: CORO1B Antibody [NB100-61040] - Human colon carcinoma. Antibody used at a dilution of 1:1,000.

Immunohistochemistry-Paraffin: CORO1B Antibody [NB100-61040] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-Coronin 2 used at a dilution of 1:250.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CORO1B Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 450 and 489 of human Coronin, actin binding protein, 1B using the numbering given in entry NP_065174.1 (GeneID 57175).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CORO1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CORO1B Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CORO1B Antibody

• coronin, actin binding protein, 1B
• coronin, actin-binding protein, 1B
• coronin-1B
• coronin-2
• DKFZp762I166

Background

Coronin 2 is an F-actin binding protein that plays an important role in processes involving actin dynamics such as fibroblast migration, and phagocytosis. Coronin 2 influences actin dynamics by promoting actin depolymerization. It has been shown to interact with the Arp2/3 complex and inhibit Arp2/3-induced actin nucleation. Additionally, it can regulate motility by regulating the actin severing and disassembling activity of cofilin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CORO1B Antibody (NB100-61040)(1)

Publication using NB100-61040

• Kimura T, Kaneko Y, Yamada S et al. The GDP-dependent Rab27a effector coronin 3 controls endocytosis of secretory membrane in insulin-secreting cell lines. J Cell Sci 2008-09-01 [PMID: 18768935]

Bioinformatics

• Gene Symbol: CORO1B
• Entrez:
  • 57175(Human)
  • 23789(Mouse)
• Uniprot:
  • Q9BR76(Human)