Coronin 3 Antibody

Western Blot: Coronin 3 Antibody [NBP1-31283] - Wild-type (WT) and Coronin 1C knockout (KO) HEK293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1C antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Coronin 3 Antibody [NBP1-31283] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: Coronin 3 Antibody [NBP1-31283] - human Sarcoma meta to brain Blood Vessel, using CORO1C antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Coronin 3 Antibody [NBP1-31283] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1C antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Coronin 3 Antibody [NBP1-31283] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1C antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Mu, Bv, RM
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Coronin 3 Antibody Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit Coronin 3 Antibody (NBP1-31283) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Coronin 3. The exact sequence is proprietary.
• Predicted Species: Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CORO1C
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Coronin 3 Antibody

• coronin 1C
• coronin, actin binding protein, 1C
• coronin, actin-binding protein, 1C
• coronin-1C
• Coronin-3
• CRNN4
• HCRNN4

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CORO1C