![ELISA: Human Complement Factor B ELISA Kit (Colorimetric) [NBP2-60564] - Standard curve.](http://images.novusbio.com/fullsize2/Human-Complement-Factor-B-ELISA-Kit-(Colorimetric)-ELISA-NBP2-60564-img0001.jpg "ELISA: Human Complement Factor B ELISA Kit (Colorimetric) [NBP2-60564] - Standard curve.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Serum, Plasma, Cell Culture, Saliva, Milk, CSF |
| Standard Curve Range | 4.375-280 ng/ml (example only; lot dependent) |
| Sensitivity | 3.9 ng/ml (example only; lot dependent) |
| Description | This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples. |
| Standard Curve Range | 4.375-280 ng/ml (example only; lot dependent) |
| Sensitivity | 3.9 ng/ml (example only; lot dependent) |
| Assay Type | Sandwich ELISA |
| Inter-Assay | CV% < 8.10% (example only; lot dependent) |
| Intra-Assay | CV% < 4.80% (example only; lot dependent) |
| Spike Recovery | 97.00% (example only; lot dependent) |
| Kit Type | ELISA Kit (Colorimetric) |
| Gene | CFB |
| Storage | Storage is content dependent. |
![Flow Cytometry: Complement C3 Antibody (11H9) [NB200-540] - Left panel: FMO, Middle panel: No primary antibody control, Right panel: sample. Day 6 murine mammary tumors processed and stained for analysis with flow cytometry. The C3b+ population of CD45+ cells is what the gate in each sample is exhibiting. WB image submitted by a verified customer review.](http://images.novusbio.com/images2/Complement-C3-Antibody-(11H9)-Flow-Cytometry-NB200-540-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.](http://images.novusbio.com/images2/Complement-C3-Antibody-(11H9)-Immunocytochemistry-Immunofluorescence-NB200-540-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse lymph node using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. This representative photomicrograph shows a membrane-cytoplasmic immunopositivity in non-germinal center cells, and few cells developed an intense staining for this target protein.](http://images.novusbio.com/images2/Complement-C3-Antibody-(11H9)-Immunohistochemistry-Paraffin-NB200-540-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C4a/b Antibody (JM88-13) [NBP2-66995] - Staining C4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Complement-C4a-b-Antibody-(JM88-13)-Immunocytochemistry-Immunofluorescence-NBP2-66995-img0001.jpg)
![Immunohistochemistry-Paraffin: Complement C4a/b Antibody (JM88-13) [NBP2-66995] - Analysis of paraffin-embedded human liver cancer tissue using anti-C4 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Complement-C4a-b-Antibody-(JM88-13)-Immunohistochemistry-Paraffin-NBP2-66995-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C4a/b Antibody (JM88-13) [NBP2-66995] - Analysis of paraffin-embedded human liver tissue using anti-C4 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Complement-C4a-b-Antibody-(JM88-13)-Immunohistochemistry-Paraffin-NBP2-66995-img0003.jpg)
![Flow Cytometry: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of Hela cells with hnRNP C1+C2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(SN0652)-Flow-Cytometry-NBP2-67656-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunocytochemistry-Immunofluorescence-NBP2-67656-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunocytochemistry-Immunofluorescence-NBP2-67656-img0003.jpg)
![Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295] - Baboon liver. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/images2/Complement-C4-Antibody-Immunohistochemistry-Paraffin-NBP3-12295-img0001.jpg)
![Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295] - Baboon kidney. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/images2/Complement-C4-Antibody-Immunohistochemistry-Paraffin-NBP3-12295-img0003.jpg)
![Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295] - Baboon liver. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/images2/Complement-C4-Antibody-Immunohistochemistry-Paraffin-NBP3-12295-img0005.jpg)
![ELISA: HLA B Antibody (2G7B10) [NBP2-61871] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/images2/HLA-B-Antibody-(2G7B10)-ELISA-NBP2-61871-img0001.jpg)
![Flow Cytometry: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of MCF-7 cells using HLA-B mouse mAb (green) and negative control (red).](http://images.novusbio.com/images2/HLA-B-Antibody-(2G7B10)-Flow-Cytometry-NBP2-61871-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of Hela cells using HLA-B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/images2/HLA-B-Antibody-(2G7B10)-Immunocytochemistry-Immunofluorescence-NBP2-61871-img0003.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the PE/Cy5 conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control PE-Cy5 (NBP1-43073) (blue histogram) or Anti-Human HLA-ABC PE-Cy5 (purple histogram). Cells in the lymphocyte gate were](http://images.novusbio.com/images2/HLA-ABC-Antibody-(W6-32)-Flow-Cytometry-NB100-64775-img0014.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the FITC conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control FITC (NBP1-43955) (blue histogram) or Anti-Human HLA-ABC FITC (purple histogram). Cells in the lymphocyte gate were used f](http://images.novusbio.com/images2/HLA-ABC-Antibody-(W6-32)-Flow-Cytometry-NB100-64775-img0013.jpg)
![Immunohistochemistry-Frozen: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis of Mouse colon cancer tissue using HLA ABC antibody (W6/32). Primary antibody dilution was 1:100. Sample was incubated with secondary antibody in 1:300 dilution. Image from verified customer review.](http://images.novusbio.com/images2/HLA-ABC-Antibody-(W6-32)-Immunohistochemistry-Frozen-NB100-64775-img0015.jpg)
![Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).](http://images.novusbio.com/images2/MHC-Class-I-Antibody-(OX18)-Flow-Cytometry-NB120-6405-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MHC Class I Antibody (OX18) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/MHC-Class-I-Antibody-(OX18)-Immunocytochemistry-Immunofluorescence-NB120-6405-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, using immunocytochemistry on rat Schwann cells (SCs). Corresponding merges are shown in the bottom rows. Treatment of SCs with IL-17 was performed at concentrations of 0.5 and 50 ng/mL. Graphs to the right show densitometry quantification. SCs showed expression of MHCI > TAPII > MHCII, which increased after IL-17 treatment. MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P <=0.01). Image collected and cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-11-63), licensed under a CC-BY licence.](http://images.novusbio.com/images2/MHC-Class-I-Antibody-(OX18)-Immunocytochemistry-Immunofluorescence-NB120-6405-img0009.jpg)
![Flow Cytometry: Frataxin Antibody (1C12) [NBP2-01743] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Frataxin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images/Frataxin-Antibody-(1C12)-Flow-Cytometry-NBP2-01743-img0001.jpg)
![Western Blot: Frataxin Antibody (1C12) [NBP2-01743] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Frataxin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Frataxin.](http://images.novusbio.com/images/Frataxin-Antibody-(1C12)-Western-Blot-NBP2-01743-img0002.jpg)
![Immunohistochemistry-Paraffin: Frataxin Antibody (1C12) [NBP2-01743] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Frataxin mouse monoclonal antibody.](http://images.novusbio.com/images/Frataxin-Antibody-(1C12)-Immunohistochemistry-Paraffin-NBP2-01743-img0003.jpg)
![Simple Western: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Simple Western lane view shows a specific band for GLO1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.](http://images.novusbio.com/images2/Glyoxalase-I-Antibody-(Glo1a)-Simple-Western-NBP1-19015-img0006.jpg)
![Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - HeLa whole cell extracts.](http://images.novusbio.com/images2/Glyoxalase-I-Antibody-(Glo1a)-Western-Blot-NBP1-19015-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb. Figure is a representative of 3 independent experiments. Photo courtesy of Dr. Nagaraj, Case Western Reserve University.](http://images.novusbio.com/images2/Glyoxalase-I-Antibody-(Glo1a)-Immunocytochemistry-Immunofluorescence-NBP1-19015-img0004.jpg)
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| Gene Symbol | CFB |
![Western Blot: Complement C4b/d Antibody (16D2) [NB200-541] - Western blot analysis of C4 expression in J774.A1 (A) aand mouse PBL (B) whole cell lysates.](http://images.novusbio.com/images2/Complement-C4b-d-Antibody-(16D2)-Western-Blot-NB200-541-img0001.jpg)
![Western Blot: FACA/FANCA Antibody [NB100-2564] - Detection of FANC A in transfected COS1 cell lysate.](http://images.novusbio.com/images2/FACA-FANCA-Antibody-Western-Blot-NB100-2564-img0004.jpg)
