Complement C4d Antibody - BSA Free

Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.

Immunohistochemistry: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of human kidney

Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Complement C4d Antibody - BSA Free Summary

• Description: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Immunogen: Synthetic peptide corresponding to aa1241-1256 of C4 conjugated to KLH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: C4A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:20-1:80
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:20-1:80
  • Immunohistochemistry-Paraffin 1:20-1:80
• Application Notes: Immunohistochemistry (Paraffin): Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining. Immunofluorescence. Optimal dilutions to be determined by the researcher.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.1% Proclin 300
• Purity: Protein G purified

Notes

May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Alternate Names for Complement C4d Antibody - BSA Free

• C4
• C4A
• C4A2
• C4A3
• C4A4
• C4A6
• C4S
• CO4
• complement C4A
• CPAMD2
• RG

Background

Complement factor C4, formerly known as Gg protein, consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the mannose binding lectin (MBL) activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. The latter can be cleaved resulting in C4c and C4d, in which step all functional sites are lost. Therefore, the C4d activation fragment of C4 is an excellent marker for classical complement pathway and MBL pathway activation. In a number of diseases such as rheumatoid arthritis (RA), hereditary angioedema (HAE), systemic lupus erythematosus (SLE) and chronic urticaria with hypercomplementemia levels of C4d are significantly elevated in serum or plasma. C4d levels may also be elevated in plasma from patients with a variety of humoral autoimmune diseases in which complement activation is known to occur. Deposition of C4d in peritubular capillaries has been shown to be a sensitive marker for antibody-mediated (humoral) rejection in renal transplant biopsies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C4A
• Uniprot:
  • P0C0L4(Human)