Complement C3c Antibody (013E-498.2.3 (10-02A)) [DyLight 488]

Product Discontinued

Complement C3c Antibody (013E-498.2.3 (10-02A)) [DyLight 488] Summary

• Immunogen: Human C3 from normal serum, highly purified by chromatography
• Specificity: NB100-64339 recognizes 135 kDa complement component 3c (C3c), present in blood serum. C3c is generated over the course of complement activation, where convertase C4b2a (classical pathway) and convertase C3bBb (alternative pathway) cleave C3 to C3b and C3a. C3b is further degraded into iC3b and C3dg/C3d. iC3b is then slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: C3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Complement C3c Antibody (013E-498.2.3 (10-02A)) [DyLight 488]

• AHUS5
• ARMD9
• ASP
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
• complement C3
• complement component 3
• complement component C3
• CPAMD1acylation-stimulating protein cleavage product

Background

C3 consists of two polypeptide chains (alpha, molecular weight 115 kDa and beta, molecular weight 75 kDa) and is usually present in serum at 1 to 2 mg/L. It is the pivotal protein of the complement system, being central in the classical pathway and the alternative pathway. It is cleaved by the classical pathway convertase C4b2a and the alternative pathway convertase C3bBb, into C3b and C3a. The C3a peptide is an important anaphylotoxin, whilst the C3b has three main roles: 1. Important opsonin phagocyte cells have C3b receptors. 2. Participates in the amplification loop in the alternative pathway. 3. Complexed with C4b2a this is the C5 convertase which leads ultimately to target cell lysis. C3c, along with iC3b and C3dg, is formed as a result of C3b inactivation by Factor I and various cofactors. The C3 fragments are eliminated by liver phagocytic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C3