Canine COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

• Reactivity: Ca
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
• Standard Curve Range: 15.6 - 1000 ng/mL
• Sensitivity: 5.6 ng/mL

Canine COMP/Thrombospondin-5 ELISA Kit (Colorimetric) Summary

• Description: Assay time: 3 hours
• Standard Curve Range: 15.6 - 1000 ng/mL
• Sensitivity: 5.6 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12
• Intra-Assay: %CV < 10
• Spike Recovery: 76 - 93%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: COMP

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Canine COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

• cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple)
• cartilage oligomeric matrix protein
• cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple)
• COMP
• EDM1
• EPD1
• MED
• MEDMGC131819
• MGC149768
• PSACH
• pseudoachondroplasia (epiphyseal dysplasia 1, multiple)
• THBS5
• Thrombospondin5
• Thrombospondin-5
• TSP5

Background

COMP is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF like and calcium binding (thrombospondin like) domains. Oligomerization results from formation of a five stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for COMP/Thrombospondin-5 ELISA Kit (NBP3-42834). (Showing 1 - 1 of 1 FAQ).

1. I have synovial fluid sample and I want to work on Western bloting can you support me ,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?
   • We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374

Bioinformatics

• Gene Symbol: COMP