Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

ELISA: Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric) [NBP2-82142] - Samples were spiked with high concentrations of Rat COMP/Thrombospondin-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric) [NBP2-82142] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Urine, Tissue Homogenate
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL

Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat COMP/Thrombospondin-5 in samples. No significant cross-reactivity or interference between Rat COMP/Thrombospondin-5 and analogues was observed.
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5%
• Intra-Assay: CV% < 5%
• Spike Recovery: average = 101% for serum; average = 99% for EDTA plasma; average = 93% for cell culture media
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: COMP

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

• cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple)
• cartilage oligomeric matrix protein
• cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple)
• COMP
• EDM1
• EPD1
• MED
• MEDMGC131819
• MGC149768
• PSACH
• pseudoachondroplasia (epiphyseal dysplasia 1, multiple)
• THBS5
• Thrombospondin5
• Thrombospondin-5
• TSP5

Background

COMP is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF like and calcium binding (thrombospondin like) domains. Oligomerization results from formation of a five stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for COMP/Thrombospondin-5 ELISA Kit (NBP2-82142)(1)

Publication using NBP2-82142

• Nian-Cih Huang, Tsorng-Shyang Yang, Prabhakar Busa, Ching-Ling Lin, Ya-Chieh Fang, Ing-Jung Chen, Chih-Shung Wong, Berardo Di Matteo Detection and Evaluation of Serological Biomarkers to Predict Osteoarthritis in Anterior Cruciate Ligament Transection Combined Medial Meniscectomy Rat Model International Journal of Molecular Sciences 2021-09-22 [PMID: 34638520]

FAQs for COMP/Thrombospondin-5 ELISA Kit (NBP2-82142). (Showing 1 - 2 of 2 FAQ).

1. I have synovial fluid sample and I want to work on Western bloting can you support me ,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?
   • We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: COMP