Collagen II Antibody [Biotin]

Product Discontinued

Collagen II Antibody [Biotin] Summary

• Immunogen: Goats were hyperimmunized with bovine type II collagen.
• Specificity: Reacts with conformational determinants on bovine type II collagen as demonstrated by ELISA. May react with type II collagen from other species. Exhibits <10% cross reactivity with collagen type I, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: COL2A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100-1:2000
  • ELISA 1:1000-1:4000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:20
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:20-1:40
  • Western Blot 1:100-1:2000
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Human and Bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Collagen II Antibody [Biotin]

• Alpha-1 type II collagen
• ANFH
• AOM
• cartilage collagen
• Chondrocalcin
• COL11A3
• COL2A1
• Collagen 2
• collagen alpha-1(II) chain
• Collagen II
• collagen II, alpha-1 polypeptide
• collagen type 2
• collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
• collagen, type II, alpha 1
• Collagen-2
• MGC131516
• SEDC

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some antibodies with denatured collagen or formalin fixed, paraffin embedded tissues. Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL2A1