Home » COL9A1 » COL9A1 Antibodies » COL9A1 Antibody

COL9A1 Antibody

 Submit a Review

Images

 
(
Enlarge)
Western Blot: COL9A1 Antibody [NBP2-92796] - Analysis of extracts of mouse skeletal muscle, using COL9A1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Western Blot: COL9A1 Antibody [NBP2-92796] - Analysis of extracts of mouse skeletal muscle, using COL9A1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug ...read more
Immunohistochemistry-Paraffin: COL9A1 Antibody [NBP2-92796] - Paraffin-embedded human stomach using COL9A1.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

COL9A1 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-270 of human COL9A1 (NP_001842.3).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL9A1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Immunohistochemistry 1:50-1:100

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for COL9A1 Antibody

  • alpha-1 polypeptide
  • collagen, type IX, alpha 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...


COMP/Thrombospondin-5
DCMP0
(2 Reviews)
(6 Publications)
Species: Hu
MATN3 Antibody
NBP1-77022
Western Blot: MATN3 Antibody [NBP1-77022] - Rat thymus tissue lysate with MATN3 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/Immunofluorescence: MATN3 Antibody [NBP1-77022] - Immunofluorescence of MATN3 in 3T3 cells with MATN3 antibody at 20 ug/mL.
Immunocytochemistry/Immunofluorescence: MATN3 Antibody [NBP1-77022] - 3T3 cells tissue with MATN3 antibody at 2.5 ug/ml.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
Collagen IX alpha
2 Antibody
NBP2-30450
Immunocytochemistry/Immunofluorescence: Collagen IX alpha 2 Antibody [NBP2-30450] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Collagen IX alpha 2 Antibody [NBP2-30450] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
(2 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
COL9A3 Antibody
NBP1-85161
Immunohistochemistry-Paraffin: COL9A3 Antibody [NBP1-85161] - Staining of human eye shows strong staining in positivity in vitreous humor.
Species: Hu
Applications: IHC, IHC-P
SLC26A2 Antibody
(3F6)
H00001836-M04
Western Blot: SLC26A2 Antibody (3F6) [H00001836-M04] - Analysis of SLC26A2 expression in transfected 293T cell line by SLC26A2 monoclonal antibody (M04), clone 3F6. Lane 1: SLC26A2 transfected lysatE (81.6 KDa). Lane 2: Non-transfected lysate.
ELISA: SLC26A2 Antibody (3F6) [H00001836-M04] - Detection limit for recombinant GST tagged SLC26A2 is 0.1 ng/ml as a capture antibody.
(3 Publications)
Species: Hu
Applications: WB, ELISA, IHC, IHC-Fr, S-ELISA
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(32 Reviews)
(645 Publications)
Species: Hu
Collagen II
Antibody (5B2.5)
NB600-844
Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Staining results for the process of differentiation induction in the RCCS environment. Collagen II immune-histochemical staining on cells after inoculation onto slides. Image collected and cropped by Cite Ab from the following publication (Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system. Cell Mol Biol Lett (2019)) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Analysis of a FFPE section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.
(21 Publications)
Species: Hu, Mu, Rt, Ch, Rb
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Aggrecan
Neoepitope Antibody
NB100-74350
Western Blot: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in L929 whole cell extract using NB100-74350.
Immunocytochemistry/Immunofluorescence: Aggrecan Neoepitope Antibody [NB100-74350] - Detection of ACAN in OA cartilage using NB100-74350.
Immunohistochemistry: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in mouse cartilage with hematoxylin counterstain.
(16 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
IL-6 [HRP]
D6050
(25 Reviews)
(463 Publications)
Species: Hu
p53 Antibody (PAb
240)
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
(3 Reviews)
(28 Publications)
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
Hairless Antibody
[Unconjugated]
AF5708
Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Hairless Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5708) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Hairless at approximately 96 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Hairless was detected in immersion fixed human NHEK epidermal keratinocytes using 10 µg/mL Goat Anti-Human Hairless Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5708) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Publication)
Species: Hu
Applications: WB, ICC
Collagen XI alpha
2 Antibody (473)
NBP2-43728
Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with COL11A2 antibody [473] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Collagen XI alpha 2 Antibody (473) [NBP2-43728] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: COL11A2 protein stained by COL11A2 antibody [473] diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human COL11A2-transfected 293T cells (N-terminal fragment of COL11A2 isoform 1) 12 % SDS-PAGE COL11A2 antibody [473] dilution: 1:5000
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
POMC Antibody
NB100-1533
Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Staining of paraffin embedded Human Pituitary Gland. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
(10 Publications)
Species: Hu, Mu, Rt, Po, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
COL11A1 Antibody
NBP2-58159
Immunocytochemistry/Immunofluorescence: COL11A1 Antibody [NBP2-58159] - Staining of human cell line BJ shows localization to endoplasmic reticulum. Antibody staining is shown in green.
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC
Hemoglobin A2
Antibody
NBP3-05569
Hemoglobin A2 Antibody
Species: Mu, Rt
Applications: WB, IHC
Catalase Antibody
NBP2-24916
Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
(1 Review)
(6 Publications)
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
Prolactin Antibody
[Unconjugated]
AF1445
Western blot shows lysates of mouse pituitary tissue and rat pituitary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Recombinant Mouse Prolactin (Catalog # 1445-PL) stimulates proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Rec­ombinant Mouse Prolactin (10 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445). The ND50 is typically 0.25-1.0 µg/mL.
(5 Publications)
Species: Mu, Rt
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
COL9A1 Antibody
NBP2-92796
COL9A1 Antibody
Species: Hu, Mu
Applications: WB, IHC

Publications for COL9A1 Antibody (NBP2-92796) (0)

There are no publications for COL9A1 Antibody (NBP2-92796).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for COL9A1 Antibody (NBP2-92796) (0)

There are no reviews for COL9A1 Antibody (NBP2-92796). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for COL9A1 Antibody (NBP2-92796) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

Additional COL9A1 Products

Bioinformatics Tool for COL9A1 Antibody (NBP2-92796)

Discover related pathways, diseases and genes to COL9A1 Antibody (NBP2-92796). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for COL9A1 Antibody (NBP2-92796)

Discover more about diseases related to COL9A1 Antibody (NBP2-92796).
 

Pathways for COL9A1 Antibody (NBP2-92796)

View related products by pathway.

PTMs for COL9A1 Antibody (NBP2-92796)

Learn more about PTMs related to COL9A1 Antibody (NBP2-92796).

Blogs on COL9A1

There are no specific blogs for COL9A1, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our COL9A1 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol COL9A1