Coagulation Factor III/Tissue Factor Antibody (S04-7G2)

Western Blot: Coagulation Factor III/Tissue Factor Antibody (S04-7G2) [NBP3-14883] - Western blot detection of Coagulation Factor III/Tissue Factor in Hela, A549, MCF-7, U251, HL-60 cell lysates using Coagulation Factor III/Tissue Factor Rabbit mAb (1:1000 diluted). Predicted band size: 33kDa. Observed band size: 45kDa.

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody (S04-7G2) [NBP3-14883] - Immunohistochemistry of Coagulation Factor III/Tissue Factor in paraffin-embedded Human lung cancer tissue using Coagulation Factor III/Tissue Factor Rabbit mAb at dilution 1/50

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S04-7G2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Coagulation Factor III/Tissue Factor Antibody (S04-7G2) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Coagulation Factor III/Tissue Factor (Uniprot#: P13726)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: F3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1000-1:2000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4), 150mM sodium chloride, 0.05% BSA, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Coagulation Factor III/Tissue Factor Antibody (S04-7G2)

• CD142 antigen
• CD142
• coagulation factor III (thromboplastin, tissue factor)
• Coagulation Factor III
• F3
• FLJ17960
• TF
• TFA
• Thromboplastin
• Tissue Factor

Background

Tissue Factor encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Coagulation Factor III/Tissue Factor Antibody (NBP3-14883). (Showing 1 - 1 of 1 FAQ).

1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
   • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

Bioinformatics

• Gene Symbol: F3