CNTFR Antibody

Western Blot: CNTFR Antibody [NB100-55418] - (0.3ug/ml) staining of mouse brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Hu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CNTFR Antibody Summary

• Immunogen: Peptide with sequence CRSNTYPKGFY corresponding to internal region according to NP_001833.1.
• Epitope: CRSNTYPKGFY
• Specificity: This antibody is expected to recognise both reported isoforms (NP_671693.1; NP_001833.1).
• Predicted Species: Human (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CNTFR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 38-40 kDa band observed in mouse brain lysates (calculated MW of 40.8 kDa band according to mouse NP_057882.1).
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CNTFR Antibody

• ciliary neurotrophic factor receptor alpha
• ciliary neurotrophic factor receptor subunit alpha
• ciliary neurotrophic factor receptor
• CNTF receptor subunit alpha
• CNTFR alpha
• CNTFR-alpha
• MGC1774

Background

This gene encodes a hematopoeitin/interferon-class receptor belonging to the cytokine superfamily of receptors. The encoded gene product represents the CNTF-specific alpha subunit of a heterotrimer forming the CNTF receptor complex, which also includes LIFR and gp130. The receptor is attached to the membrane by a glycosyl-phosphatidylinositol linkage and contains an immunoglobulin-like C2-type domain and a fibronectin type-III domain. Signal transduction requires that CNTF bind first to this alpha component, which permits the recruitment of gp130 and LIFR beta to form the tripartite receptor complex. Signal transduction stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types. Alternative splicing has been observed at this locus and two variants, both encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CNTFR Antibody (NB100-55418)(1)

Publication using NB100-55418

• Yokota H, Yoshikawa M, Hirabayashi H et al. Expression of ciliary neurotrophic factor (CNTF), CNTF receptor alpha (CNTFR-alpha) following experimental intracerebral hemorrhage in rats. Neurosci Lett 2005-04-04 [PMID: 15755520]

Bioinformatics

• Gene Symbol: CNTFR
• Entrez:
  • 1271(Human)
  • 12804(Mouse)
  • 313173(Rat)
• Uniprot:
  • P26992()