Recombinant Mouse CNTF Protein

SDS-Page: Mouse CNTF Protein [NBP2-35168]

• Reactivity: Mu
• Applications: Func, PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse CNTF Protein Summary

• Description: A biologically active protein to CNTF.
  
  Source: E. coli
  
  Amino Acid Sequence: AFAEQSPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNISLDSVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPTEGDFHQAIHT LTLQVSAFAY QLEELMALLE QKVPEKEADG MPVTIGDGGLFEKKLWGLKV LQELSQWTVR SIHDLRVISS HHMGISAHES HYGAKQM
• Preparation Method: Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
• Details of Functionality: CNTF Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x 10^4 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CNTF
• Purity: >95% pure by SDS-PAGE
• Endotoxin Note: Less than 1 EU/ug of endotoxin as determined by LAL method

Applications/Dilutions

• Theoretical MW: 22.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4, 2 % trehalose.
• Preservative: Trehalose
• Concentration: LYOPH
• Purity: >95% pure by SDS-PAGE
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Mouse CNTF Protein

• ciliary neurotrophic factor
• CNTF
• HCNTF

Background

Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Furthermore, the protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition, CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress. Recombinant murine CNTF containing 197 amino acids and it shares 82 % and 95 % a.a. sequence identity with human and rat CNTF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for CNTF Protein (NBP2-35168). (Showing 1 - 1 of 1 FAQ).

1. Is mouse CNTF species specific?
   • With regard to our mouse CNTF protein NBP2-35168, this particular protein is made to the mouse CNTF sequence. The functionality of this protein has only been investigated as a mouse protein. We have not yet tested this protein with use in rat samples or with rat specific antibodies. In general, the rat and mouse sequences for CNTF are very similar. Given an antibody made specifically to the mouse sequence, it is likely to cross react with the rat protein. However, with a protein product, there can be marked differences in the folding and activity of the protein that would prohibit it from being used in a species other than intended.

Bioinformatics

• Gene Symbol: CNTF