Reactivity | MuSpecies Glossary |
Applications | Func, PAGE, Bioactivity |
Format | Carrier-Free |
Concentration | LYOPH |
Description | A biologically active protein to CNTF. Source: E. coli Amino Acid Sequence: AFAEQSPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNISLDSVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPTEGDFHQAIHT LTLQVSAFAY QLEELMALLE QKVPEKEADG MPVTIGDGGLFEKKLWGLKV LQELSQWTVR SIHDLRVISS HHMGISAHES HYGAKQM |
Preparation Method |
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance. |
Details of Functionality | CNTF Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x 10^4 IU/mg. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | CNTF |
Purity | >95% pure by SDS-PAGE |
Endotoxin Note | Less than 1 EU/ug of endotoxin as determined by LAL method |
Theoretical MW | 22.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at -20C. Avoid freeze-thaw cycles. |
Buffer | Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4, 2 % trehalose. |
Preservative | Trehalose |
Concentration | LYOPH |
Purity | >95% pure by SDS-PAGE |
Reconstitution Instructions | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Diseases for CNTF Protein (NBP2-35168)Discover more about diseases related to CNTF Protein (NBP2-35168).
| Pathways for CNTF Protein (NBP2-35168)View related products by pathway.
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PTMs for CNTF Protein (NBP2-35168)Learn more about PTMs related to CNTF Protein (NBP2-35168).
| Research Areas for CNTF Protein (NBP2-35168)Find related products by research area.
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Gene Symbol | CNTF |