CNTF Antibody [Unconjugated]

CNTF was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat CNTF Polyclonal Antibody (Catalog # AB-557-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown); (CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of glial cells in the white matter. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Recombinant Rat CNTF (Catalog # 557-NT) supports the survival of dorsal root ganglion neurons from E10 chick embryos in a dose-dependent manner (orange line), as measured by MTT staining. Neuron survival elicited by Recombinant Rat CNTF (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Polyclonal Antibody (Catalog # AB-557-NA). The ND50 is typically 7-15 µg/mL.

• Reactivity: Rt
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CNTF Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant rat CNTF
  Ala2-Met200
  Accession # P20294
• Specificity: Detects rat CNTF in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CNTF
• Purity Statement: Protein A or G purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 7-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CNTF Antibody [Unconjugated]

• ciliary neurotrophic factor
• CNTF
• HCNTF

Background

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons, and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CNTF Antibody (AB-557-NA)(5)

Publications using AB-557-NA

• C Jia, W Drew Gill, C Lovins, RW Brown, T Hagg Female-specific role of ciliary neurotrophic factor in the medial amygdala in promoting stress responses Neurobiology of stress, 2022-01-31;17(0):100435. 2022-01-31 [PMID: 35146079] (Neutralization, Mouse)
• Min‐Tae Jeon, Gyeong Joon Moon, Sehwan Kim, Minji Choi, Yong‐Seok Oh, Dong Woon Kim et al. Neurotrophic interactions between neurons and astrocytes following AAV1‐Rheb(S16H) transduction in the hippocampus in vivo British Journal of Pharmacology 2020-02-01 [PMID: 31658360]
• Nan Gao, Chenxi Yan, Patrick Lee, Haijing Sun, Fu-Shin Yu Dendritic cell dysfunction and diabetic sensory neuropathy in the cornea Journal of Clinical Investigation 2016-04-11 [PMID: 27064280]
• Julien Vernerey, Magali Macchi, Karine Magalon, Myriam Cayre, Pascale Durbec Ciliary Neurotrophic Factor Controls Progenitor Migration during Remyelination in the Adult Rodent Brain The Journal of Neuroscience 2013-02-13 [PMID: 23407977]
• Yang P, Arnold SA, Habas A, Hetman M, Hagg T Ciliary neurotrophic factor mediates dopamine D2 receptor-induced CNS neurogenesis in adult mice. J. Neurosci., 2008-02-27;28(9):2231-41. 2008-02-27 [PMID: 18305256] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: CNTF
• Uniprot:
  • P20294()