CNTF Antibody (21809) [Unconjugated]

Recombinant Human CNTF (Catalog # 257-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CNTF (400 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CNTF Monoclonal Antibody (Catalog # MAB657). The ND50 is typically 2-6 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 21809
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CNTF Antibody (21809) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CTNF
  Ala2-Met200
  Accession # P26441.1
• Specificity: Detects human CNTF in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CNTF
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 2-6 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human CNTF Biotinylated Antibody (Catalog number BAF257)
  Standard: Recombinant Human CNTF (Catalog number 257-NT)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CNTF Antibody (21809) [Unconjugated]

• ciliary neurotrophic factor
• CNTF
• HCNTF

Background

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for human CNTF encodes a 200 amino acid polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNTF
• Uniprot:
  • P26441.1()