Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, mouse thymus tissue, and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse CMG‑2/ANTXR2 Antigen Affinity-purified ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Immunogen affinity purified
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for CMG-2/ANTXR2 Antibody
anthrax toxin receptor 2
Capillary morphogenesis gene 2 protein
capillary morphogenesis protein 2
Capillary Morphogenesis Gene-2 (CMG-2) is a widely expressed anthrax toxin receptor (ATR) family protein (1‑4). CMG-2 is a ~55 kDa protein that contains a 31 amino acid (aa) signal sequence, a 287 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 148 aa cytoplasmic domain. Unlike human CMG-2 which has four isoforms, only one sequence has been reported for mouse CMG-2. The main functional domain of CMG‑2 is an extracellular integrin-like von Willebrand factor type A (VWA) domain with a metal ion dependent adhesion site (MIDAS), through which it adheres selectively to collagen type IV and laminin (1‑6). CMG-2 isoform 2 is induced in HUVEC as they undergo capillary formation in collagen matrices in vitro (4). In humans, CMG-2 is mutated in juvenile hyaline fibromatosis and infantile systemic hyalinosis disorders, and several of these mutations result in loss of laminin binding (7). CMG-2 and the related protein ATR/TEM8 serve as receptors for the protective antigen (PA) of Bacillus anthracis (1, 2). After binding the VWA domain, PA undergoes furin-type cleavage, forms a heptameric receptor/PA pre-pore and binds LF or EF toxin subunits (6, 8, 9). Transport to low pH endosomes, which requires CMG-2 ubiquitination and interaction with the LDL receptor related protein LRP6 (10, 11), allows PA pore formation and release of toxin to the cytoplasm (11, 12). Soluble CMG-2 VWA domain acts as a dummy receptor that can protect cultured cells from anthrax intoxication (2). Within the extracellular region, mouse CMG-2 shares 84%, 91%, 80%, and 83% amino acid sequence homology with human, rat, bovine, and canine CMG-2, respectively. CMG-2 VWA domain also shares 60% aa identity with ATR/TEM8.
Scobie, H.M. and J.A.T. Young (2005), Curr. Opin. Microbiol. 8:106.
Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170.
Swiss Prot. Accession #: Q6DFX2.
Bell, S.E. et al. (2001) J. Cell Sci. 114:2755.
Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6367.
Santelli, E. et al. (2004) Nature 430:905.
Dowling, O. et al. (2003) Am. J. Hum. Genet. 73:957.
Wigelsworth, D.J. et al. (2004) J. Biol. Chem. 279:23349.
Go, M.Y. et al. (2006) J. Mol. Biol. 360:145.
Abrami, L. et al. (2006) J. Cell Biol. 172:309.
Wei, W. et al. (2006) Cell 124:1141.
Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13147.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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