CMG-2/ANTXR2: Novus Biologicals

AF2940

CMG-2/ANTXR2 Antibody [Unconjugated]

100 μg

AF2940

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox">

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." alt="Human whole blood monocytes were stained with Human CMG-2/ANTXR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2940, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />

<b>Biological Strategies Validation. </b>Number and localization of glycan sidechains determine trafficking efficiency of TEM8 and CMG2.A) Endoglycosidase H (EndoH) treatment on TEM8 and CMG2 single mutants. HeLa cells were transfected for 48h with the respective cDNAs. 40 μg of cell extracts were treated or not with EndoH as described before. Samples were analyzed by SDS-PAGE and Western Blotting. B) Quantification of surface biotinylation experiments to determine amount of TEM8 at the cell surface. All mutants were corrected for their expression levels and then normalized to WT, which was set at 100%. Errors represent standard deviation. Statistics were calculated using an unpaired t-test. n ≥ 3. * p≤0.05, ** p≤0.01, *** p≤0.001 C) Representative Western Blots of surface biotinylation. HeLa cells were transfected 48h with the respective cDNAs. Proteins at the cell surface were labeled with biotin, immunoprecipitated with streptavidin beads and blotted against TEM8-HA. D) Quantification of surface biotinylation experiments to determine amount of CMG2 at the cell surface. All mutants were corrected for their expression levels and then normalized to WT, which was set at 100%. Errors represent standard deviation. Statistics were calculated using an unpaired t-test. n ≥ 3. * p≤0.05, ** p≤0.01, *** p≤0.001 E) Representative Western Blots of surface biotinylation. HeLa cells were transfected 48h with the respective cDNAs. Proteins at the cell surface were labeled with biotin, immunoprecipitated with streptavidin beads and blotted against CMG2-V5. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25781883), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Glycosylation acts as a buffer for CMG2 ectodomain mutations.A) Graphic showing the disulfide bridge C39-C218 (blue) present in CMG2 WT B) Fibroblast cells were treated or not with tunicamycin for 16h and TCE were analyzed by SDS-PAGE and Western Blot. Representative Western Blot with control fibroblasts and patient fibroblasts. Calnexin serves as a loading control. C) Quantification of total protein levels. CMG2 levels are normalized to WT protein level without tunicamycin treatment, which was set to 100%. Statistics were calculated using an unpaired t-test. Errors represent standard deviation. n ≥ 3. * p≤0.05 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25781883), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Loss of glycosylation affects binding of Anthrax toxin to TEM8 but not to CMG2.A and C) HeLa cells were transfected for 48h with the respective cDNAs. Cells were treated for 1h at 4°C with 500 ng/ml PA83 and shifted to 37°C for 10 min to induce cleavage and heptamerization. Immunoprecipitates against TEM8-HA/CMG2-V5 were analyzed by SDS-PAGE and Western Blotting against PA and TEM8-HA/CMG2-V5. Control cells are non-transfected. D50A is a binding deficient mutant that serves as a negative control. B and D) HeLa cells were transfected for 48h with the respective cDNAs. Cells were lysed and incubated for 1h at 4°C with 1 μg/ml PA83. Immunoprecipitates against TEM8-HA/CMG2-V5 were analyzed by SDS-PAGE and Western Blotting against PA and TEM8-HA/CMG2-V5. Control cells are non-transfected. D50A is a binding deficient mutant that serves as a negative control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25781883), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>CMG2 and TEM8 can undergo N-glycosylation on all predicted sites.A) Graphics depicting glycosylation sites on TEM8 and CMG2. Sites in red are unique to the respective proteins, N260 in CMG2 (yellow) corresponds to N262 in TEM8. B) Expression of TEM8 glycosylation mutants in HeLa cells. Cells were transfected for 48h with the respective cDNAs. Expression was analyzed by SDS-PAGE and Western Blotting. C) Expression of all CMG2 glycosylation mutants in HeLa cells. Cells were transfected for 48h with the respective cDNAs. Expression was analyzed by SDS-PAGE and Western Blotting. D) Endoglycosidase F (NGaseF) treatment on TEM8 glycosylation mutants. 40 μg of cell extracts were treated or not with NGaseF and analyzed by SDS-PAGE and Western Blotting. E) Endoglycosidase F (NGaseF) treatment on CMG2 glycosylation mutants. 40 μg of cell extracts were treated or not with NGaseF and analyzed by SDS-PAGE and Western Blotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25781883), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Non-glycosylated TEM8 is an ER quality control and ERAD substrate.A) HeLa cells were transfected for 48h with the respective cDNAs. Cells were treated or not with MG132, an inhibitor of the proteasome or Bafilomycin A1, a drug preventing endosomal acidification and thus lysosomal degradation. Immunoprecipitates against TEM8-HA were analyzed by SDS-PAGE and Western Blotting against Ubiquitin and TEM8-HA. B) HEK cells stably expressing CMG2 under the control of a tetracycline inducible promotor were induced for 24h with 0.1μg/ml doxycycline. Cells were treated or not with MG132 or Bafilomycin A1. Immunoprecipitates against CMG2-V5 were analyzed by SDS-PAGE and Western Blotting against Ubiquitin and CMG2-V5. C) HeLa cells were treated or not with tunicamycin, an antibiotic blocking the co-translational transfer of glycan sidechains in the ER by blocking the oligosaccharyltransferase (OST) for 16h. Surface proteins were labeled with biotin and immunoprecipitates against streptavidin were analysed for TEM8 or Calnexin as a negative control. D) RpeI cells were treated or not with tunicamycin for 16h. Surface proteins were labeled with biotin and immunoprecipitates against streptavidin were analysed for CMG2 or Calnexin as a negative control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25781883), licensed under a CC-BY license. Not internally tested by R&D Systems.

2940-CM) was serially diluted and captured by Mouse Anti-Human CMG‑2/ANTXR2 Monoclonal Antibody (Catalog # MAB29401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CMG‑2/ANTXR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2940) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human CMG‑2/ANTXR2 (Catalog # <a class=

2940-CM) was serially diluted and captured by Mouse Anti-Human CMG‑2/ANTXR2 Monoclonal Antibody (Catalog # MAB29401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CMG‑2/ANTXR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2940) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2940U-100UG
Alexa Fluor 405	AF2940V-100UG
Alexa Fluor 488	AF2940G-100UG
Alexa Fluor 532	AF2940X-100UG
Alexa Fluor 594	AF2940T-100UG
Alexa Fluor 647	AF2940R-100UG
Alexa Fluor 700	AF2940N-100UG
Alexa Fluor 750	AF2940S-100UG
Alexa Fluor Plus 405	AF2940AFP405-100UG
Alexa Fluor Plus 488	AF2940AFP488-100UG
Alexa Fluor Plus 555	AF2940AFP555-100UG
Alexa Fluor Plus 594	AF2940AFP594-100UG
Alexa Fluor Plus 647	AF2940AFP647-100UG
Alexa Fluor Plus 680	AF2940AFP680-100UG
Allophycocyanin	FAB2940A
Biotin	BAF2940
CoraFluor 1	AF2940CL1
DyLight 350	AF2940D
DyLight 405	AF2940E
DyLight 488	AF2940K
DyLight 550	AF2940L
DyLight 594	AF2940M
DyLight 650	AF2940W
DyLight 680	AF2940Y
DyLight 755	AF2940Z
FITC	AF2940F
HRP	AF2940H
Janelia Fluor 525	AF2940JF525
Janelia Fluor 549	AF2940I
Janelia Fluor 646	AF2940J
Janelia Fluor 669	AF2940JF669
PerCP	AF2940C
mFluor Violet 450 SE	AF2940MFV450
mFluor Violet 500 SE	AF2940MFV500
mFluor Violet 610 SE	AF2940MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB2940A
Carrier Free	AF2940-SP

compare

(select up to 3 products from this page to compare)

AF3636

CMG-2/ANTXR2 Antibody

(1 Publication)

100 μg

AF3636

HAF019). A specific band was detected for CMG-2/ANTXR2 at approximately 55-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox">

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, mouse thymus tissue, and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse CMG-2/ANTXR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3636) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for CMG-2/ANTXR2 at approximately 55-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, mouse thymus tissue, and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse CMG-2/ANTXR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3636) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CMG-2/ANTXR2 at approximately 55-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3636U-100UG
Alexa Fluor 405	AF3636V-100UG
Alexa Fluor 488	AF3636G-100UG
Alexa Fluor 532	AF3636X-100UG
Alexa Fluor 594	AF3636T-100UG
Alexa Fluor 647	AF3636R-100UG
Alexa Fluor 700	AF3636N-100UG
Alexa Fluor 750	AF3636S-100UG
Alexa Fluor Plus 405	AF3636AFP405-100UG
Alexa Fluor Plus 488	AF3636AFP488-100UG
Alexa Fluor Plus 555	AF3636AFP555-100UG
Alexa Fluor Plus 594	AF3636AFP594-100UG
Alexa Fluor Plus 647	AF3636AFP647-100UG
Alexa Fluor Plus 680	AF3636AFP680-100UG
Allophycocyanin	AF3636A
Biotin	AF3636B
CoraFluor 1	AF3636CL1
DyLight 350	AF3636D
DyLight 405	AF3636E
DyLight 488	AF3636K
DyLight 550	AF3636L
DyLight 594	AF3636M
DyLight 650	AF3636W
DyLight 680	AF3636Y
DyLight 755	AF3636Z
FITC	AF3636F
HRP	AF3636H
Janelia Fluor 525	AF3636JF525
Janelia Fluor 549	AF3636I
Janelia Fluor 646	AF3636J
Janelia Fluor 669	AF3636JF669
PE	AF3636P
PerCP	AF3636C
mFluor Violet 450 SE	AF3636MFV450
mFluor Violet 500 SE	AF3636MFV500
mFluor Violet 610 SE	AF3636MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3636-SP

compare

(select up to 3 products from this page to compare)

2940-CM

Recombinant Human CMG-2 Protein, CF

(1 Publication)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

compare

(select up to 3 products from this page to compare)

NBP1-49866

CMG-2/ANTXR2 Antibody

(1 Publication)

0.1 mg

NBP1-49866

Reactivity:

Hu, Mu, Rt, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

3636-CM

Recombinant Mouse CMG-2 Protein

50 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	3636-CM-050/CF

compare

(select up to 3 products from this page to compare)

H00118429-B01P

CMG-2/ANTXR2 Antibody - Azide and BSA Free

0.05 mg

H00118429-B01P

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Polyclonal

Host: Mouse Polyclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP2-68222

Mouse CMG-2/ANTXR2 ELISA Kit (Colorimetric)

1 Kit

NBP2-68222

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Standard Curve:

31.25 - 2000 pg/mL

Sensitivity:

18.75 pg/mL

Sample Type:

Serum, plasma and other biological fluids

compare

(select up to 3 products from this page to compare)

NBP3-41870

CMG-2/ANTXR2 Antibody (C2) - BSA Free

100 ul

NBP3-41870

Reactivity:

Hu, Po

Species Glossary

Reactivity: Hu, Po

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP3-41856

CMG-2/ANTXR2 Antibody (C1) - BSA Free

100 ul

NBP3-41856

Reactivity:

Hu, Po

Species Glossary

Reactivity: Hu, Po

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP1-68911

CMG-2/ANTXR2 Antibody - BSA Free

100 ul

NBP1-68911

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBL1-07555

CMG-2/ANTXR2 Overexpression Lysate

0.1 mg

NBL1-07555

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

compare

(select up to 3 products from this page to compare)

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Has Sample Size

Promotional Item

Species Glossary

ANTXR2 Products By Gene Name

CMG-2/ANTXR2 Antibody [Unconjugated]

CMG-2/ANTXR2 Antibody

Recombinant Human CMG-2 Protein, CF

CMG-2/ANTXR2 Antibody

Recombinant Mouse CMG-2 Protein

CMG-2/ANTXR2 Antibody - Azide and BSA Free

Mouse CMG-2/ANTXR2 ELISA Kit (Colorimetric)

CMG-2/ANTXR2 Antibody (C2) - BSA Free

CMG-2/ANTXR2 Antibody (C1) - BSA Free

CMG-2/ANTXR2 Antibody - BSA Free

CMG-2/ANTXR2 Overexpression Lysate