Cleavage stimulation factor 2 Antibody - BSA Free

Western Blot: Cleavage stimulation factor 2 Antibody [NBP2-15915] - Sample (30 ug of whole cell lysate) A: JurKat 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Cleavage stimulation factor 2 Antibody [NBP2-15915] - HeLa cells were fixed in iced-cold MeOH for 5 min. Green: CSTF2 protein stained by CSTF2 antibody, N-term diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry-Paraffin: Cleavage stimulation factor 2 Antibody [NBP2-15915] - Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cleavage stimulation factor 2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Cleavage stimulation factor 2. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSTF2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Cleavage stimulation factor 2 Antibody - BSA Free

• betaCstF-64 variant 2
• CF-1 64 kDa subunit
• Cleavage stimulation factor 64 kDa subunit
• cleavage stimulation factor subunit 2
• cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kD
• cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
• CSTF 64 kDa subunit
• CstF-64

Background

This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CSTF2
• Uniprot:
  • P33240(Human)