CSTF2T Antibody

Images

 
Western Blot: CSTF2T Antibody [NB100-74614] - Detection of Human CSTF2T by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit ...read more
Immunohistochemistry: CSTF2T Antibody [NB100-74614] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CSTF2T/TauCSTF64 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: CSTF2T Antibody [NB100-74614] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74614 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 ...read more
Immunohistochemistry-Paraffin: CSTF2T Antibody [NB100-74614] - FFPE section of human skin squamous cell carcinoma. NB100-74614 used at a dilution of 1:250. DAB staining using Immunohistochemistry Accessory Kit
Immunohistochemistry-Paraffin: CSTF2T Antibody [NB100-74614] - Human skin basal cell carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

CSTF2T Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 566 and 616 of human cleavage stimulation factor 64 kDa subunit, tau variant using the numbering given in entry NP_056050.1 (GeneID 23283).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CSTF2T
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CSTF2T Antibody

  • CF-1 64 kDa subunit tau variant
  • CF-1 64 kDa subunit tau
  • Cleavage stimulation factor 64 kDa subunit tau variant
  • cleavage stimulation factor 64 kDa subunit tau
  • cleavage stimulation factor subunit 2 tau variant
  • cleavage stimulation factor subunit 2 tau
  • cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, tau variant
  • CSTF 64 kDa subunit tau variant
  • CstF-64T
  • DKFZp434C1013
  • KIAA0689CSTF 64 kDa subunit tau
  • tauCstF-64

Background

CSTF2T, also known as tauCSTF-64, is a paralog of CstF-64, a component of the cleavage stimulation factor (CstF) that processes 3'-end formation and polyadenylation of mRNAs. The human CSTF2T/TauCSTF64 gene is located at 10q22-23, a site associated with neurological problems and male infertility. CSTF2T is expressed in all tissues and throughout meiosis in male germ cells. CstF-64 is also expressed ubiquitously; however because it is located on the X chromosome, its expression is temporarily silenced in germ cells by meiotic sex chromosome inactivation. During this inactivation, it is hypothesized that CSTF2T/TauCSTF64 can compensate for the loss of CstfF-64 expression. Recently, targeted disruption of CSTF2T/TauCSTF64 in mice has been shown to cause aberrant spermatogenesis and infertility providing more evidence for its importance in spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CSTF2T
Entrez
Uniprot