CSTF2T Antibody - BSA Free

Western Blot: CSTF2T Antibody [NB100-74614] - Detection of Human CSTF2T by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-CSTF2T antibody NB100-74614 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry: CSTF2T Antibody [NB100-74614] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CSTF2T/TauCSTF64 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: CSTF2T Antibody [NB100-74614] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74614 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CSTF2T/TauCSTF64 was also immunoprecipitated by rabbit anti-CSTF2T/TauCSTF64 antibody NB100-74613, which recognizes an upstream epitope.

Immunohistochemistry-Paraffin: CSTF2T Antibody [NB100-74614] - FFPE section of human skin squamous cell carcinoma. NB100-74614 used at a dilution of 1:250. DAB staining using Immunohistochemistry Accessory Kit

Immunohistochemistry-Paraffin: CSTF2T Antibody [NB100-74614] - Human skin basal cell carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CSTF2T Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 566 and 616 of human cleavage stimulation factor 64 kDa subunit, tau variant using the numbering given in entry NP_056050.1 (GeneID 23283).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSTF2T
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CSTF2T Antibody - BSA Free

• CF-1 64 kDa subunit tau variant
• CF-1 64 kDa subunit tau
• Cleavage stimulation factor 64 kDa subunit tau variant
• cleavage stimulation factor 64 kDa subunit tau
• cleavage stimulation factor subunit 2 tau variant
• cleavage stimulation factor subunit 2 tau
• cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, tau variant
• CSTF 64 kDa subunit tau variant
• CstF-64T
• DKFZp434C1013
• KIAA0689CSTF 64 kDa subunit tau
• tauCstF-64

Background

CSTF2T, also known as tauCSTF-64, is a paralog of CstF-64, a component of the cleavage stimulation factor (CstF) that processes 3'-end formation and polyadenylation of mRNAs. The human CSTF2T/TauCSTF64 gene is located at 10q22-23, a site associated with neurological problems and male infertility. CSTF2T is expressed in all tissues and throughout meiosis in male germ cells. CstF-64 is also expressed ubiquitously; however because it is located on the X chromosome, its expression is temporarily silenced in germ cells by meiotic sex chromosome inactivation. During this inactivation, it is hypothesized that CSTF2T/TauCSTF64 can compensate for the loss of CstfF-64 expression. Recently, targeted disruption of CSTF2T/TauCSTF64 in mice has been shown to cause aberrant spermatogenesis and infertility providing more evidence for its importance in spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSTF2T
• Entrez:
  • 23283(Human)
• Uniprot:
  • NP_056050.1(Human)