Aly Antibody (HL3813)

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Aly antibody [HL3813] (NBP3-48848) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Aly antibody [HL3813] (NBP3-48848) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Aly antibody [HL3813] detects Aly protein by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.Aly stained by Aly antibody [HL3813] (NBP3-48848) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Aly antibody [HL3813] detects Aly protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Aly stained by Aly antibody [HL3813] (NBP3-48848) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Aly antibody [HL3813] detects Aly protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Aly stained by Aly antibody [HL3813] (NBP3-48848) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Aly antibody [HL3813] detects Aly protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.Aly stained by Aly antibody [HL3813] (NBP3-48848) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Aly antibody [HL3813] detects Aly protein by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.Aly stained by Aly antibody [HL3813] (NBP3-48848) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Aly antibody [HL3813] detects Aly protein by immunohistochemical analysis.Sample: Paraffin-embedded human colon.Aly stained by Aly antibody [HL3813] (NBP3-48848) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: HL3813
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Aly Antibody (HL3813) Summary

• Description: Novus Biologicals Rabbit Aly Antibody (HL3813) (NBP3-48848) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Aly.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ALYREF
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Aly Antibody (HL3813)

• Ally of AML-1 and LEF-1
• ALY/REF
• ALYBEFbZIP enhancing factor
• bZIP-enhancing factor BEF
• REF
• THO complex 4
• THO complex subunit 4
• tho4
• Transcriptional coactivator Aly/REF

Background

Pre-mRNA splicing plays an important role in regulation of gene expression. During the splicing process, specific proteins are recruited to the mRNA and assembled into a complex of mRNA-protein near the exon-exon junction. This complex is termed the mRNA-protein complex (mRNP) or the exon-exon junction complex. The proteins that are found in this complex include: Y14, magoh, Aly/REF, RNPS1, Upf3, and DEK.1-5 Aly (also known as mREF1-I) is the mammalian homologue of the yeast mRNA export factor Yra1p.6-7 It is a 233 amino acid RNAbinding protein that contains a RNA-binding domain and is recruited to the mRNP complexes during the splicing. Excess of recombinant expression of Aly in the cells increases both the rate and efficiency of spliced and unspliced mRNA export (in vivo) from the nucleus to the cytoplasm. In contrast to the Y14 protein, Aly is dissociated from the mRNAs after the export to the cytoplasm.1, 5-6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALYREF