cleavage stimulation factor Antibody

Images

 
Western Blot: cleavage stimulation factor Antibody [NB100-60443] - Detection of Human and Mouse CSTF50 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN ...read more
Immunohistochemistry: cleavage stimulation factor Antibody [NB100-60443] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-CSTF50 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: cleavage stimulation factor Antibody [NB100-60443] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. NB100-60443 ...read more
Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443] - FFPE section of human ovarian carcinoma. NB100-60443 used at a dilution of 1:250.
Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443] - Human bladder cell carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
Immunohistochemistry: cleavage stimulation factor Antibody [NB100-60443] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CSTF50 used at a dilution of 1:1,000 (1ug/ml). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

cleavage stimulation factor Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa using the numbering given in entry NP_001315.1 (GeneID 1477 ).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CSTF1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Positive Control
cleavage stimulation factor Lysate (NBL1-09555)
Reviewed Applications
Read 1 Review rated 5
using
NB100-60443 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for cleavage stimulation factor Antibody

  • CF-1 50 kDa subunit
  • Cleavage stimulation factor 50 kDa subunit
  • cleavage stimulation factor subunit 1
  • cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kD
  • cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
  • CSTF 50 kDa subunit
  • CstF-50
  • CstFp50

Background

This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for cleavage stimulation factor Antibody (NB100-60443) (0)

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Review for cleavage stimulation factor Antibody (NB100-60443) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-60443:
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Images Ratings Applications Species Date Details
Western Blot cleavage stimulation factor NB100-60443
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reviewed by:
Anonymous
WB Human 05/22/2015
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
LotA1

Blocking

Blocking Details5% skim milk/TBS, 1h at r.t.

Primary Anitbody

Dilution Ratio1:1000, O/N, 4C, 5% skim milk in TBS

Secondary Antibody

Secondary Descriptionanti-rabbit IgG, HRP
Secondary Concentration1:10000

Details

Detection NotesECL, 30sec-1.5min exposure

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for cleavage stimulation factor Antibody (NB100-60443) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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2
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1
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Anonymous
05/22/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CSTF1
Entrez
Uniprot