![Western Blot: cleavage stimulation factor Antibody [NB100-60443] - Detection of human and mouse CSTF50 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSTF50 antibody NB100-60443 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/cleavage-stimulation-factor-Antibody-Western-Blot-NB100-60443-img0009.jpg "Western Blot: cleavage stimulation factor Antibody [NB100-60443] - Detection of human and mouse CSTF50 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSTF50 antibody NB100-60443 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, Mu, Rt, Pm-OrSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa using the numbering given in entry NP_001315.1 (GeneID 1477 ). |
| Predicted Species | Rat (100%), Orangutan (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CSTF1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| Reviewed Applications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Human | 05/22/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for cleavage stimulation factor Antibody (NB100-60443)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CSTF1 |
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![Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CSTF50 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/cleavage-stimulation-factor-Antibody-Immunohistochemistry-Paraffin-NB100-60443-img0013.jpg "Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CSTF50 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB")
![Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443] - Detection of mouse CSTF50 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CSTF50 (NB100-60443). Detection: DAB](http://images.novusbio.com/fullsize/cleavage-stimulation-factor-Antibody-Immunohistochemistry-NB100-60443-img0010.jpg "Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443] - Detection of mouse CSTF50 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CSTF50 (NB100-60443). Detection: DAB")
![Immunoprecipitation: cleavage stimulation factor Antibody [NB100-60443] - Detection of human CSTF50 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSTF50 antibody NB100-60443 used for IP at 6 ug per reaction. CSTF50 was also immunoprecipitated by another rabbit anti-CSTF50 antibody. For blotting immunoprecipitated CSTF50, NB100-60443 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/cleavage-stimulation-factor-Antibody-Immunoprecipitation-NB100-60443-img0011.jpg "Immunoprecipitation: cleavage stimulation factor Antibody [NB100-60443] - Detection of human CSTF50 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSTF50 antibody NB100-60443 used for IP at 6 ug per reaction. CSTF50 was also immunoprecipitated by another rabbit anti-CSTF50 antibody. For blotting immunoprecipitated CSTF50, NB100-60443 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
