Western Blot: Claudin-18 Antibody [NBP1-60010] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, ...read more
Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP1-60010] - Claudin-18 antibody used at 1:50 concentration in casein in PBS and left at 4C overnight on paraffin embedded canine bladder tissue. HIER was performed ...read more
Western Blot: Claudin-18 Antibody [NBP1-60010] - Tissue:Jurkat cells. Lane A: Primary Antibody. Lane B: Primary Antibody + Blocking Peptide.
Synthetic peptides corresponding to CLDN18(claudin 18) The peptide sequence was selected from the C terminal of CLDN18. Peptide sequence PEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDE. The peptide sequence for this immunogen was taken from within the described region.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
This is a rabbit polyclonal antibody against CLDN18 and was validated on Western Blot and immunohistochemistry-paraffin
28 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 4 using NBP1-60010 in the following applications:
Canine reactivity reported from a verified customer review.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
PBS & 2% Sucrose.
0.09% Sodium Azide
Immunogen affinity purified
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Alternate Names for Claudin-18 Antibody
surfactant associated 5
surfactant associated protein J
surfactant, pulmonary associated protein J
CLDN18 belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells.CLDN18 belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells (Niimi et al., 2001).[supplied by OMIM].
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Claudin-18 Antibody (NBP1-60010). (Showing 1 - 1 of 1 FAQs).
Our customer purchased NBP1-60010 for IHC. She tested as following way: Sample: human stoma cancer tissue, Dilution: 1:100 incubation, overnight used vector ABC kit. But, she got very weak stain result. So, she asked the positive tissue for this item. Could you let me know?
The Human Protein Atlas shows where tissues have stained positive using another company's antibodies for Claudin 18 (Protein Atlas). I would recommend a tissue that has been shown to stain positive from this list as a positive control. This protein is also highly expressed in the epithelial cells of the GI-tract.
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