Reg4 Antibody [Unconjugated]

Images

 
Reg4 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Reg4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1379) at 10 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Reg4 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Reg4
Asp23-Pro158
Accession # Q9BYZ8
Specificity
Detects human Reg4 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse (rm) Reg4, rmReg3A, and recombinant rat (rr) Reg3A is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
REG4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF1379 in the following applications:

Publications
Read Publications using
AF1379 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Reg4 Antibody [Unconjugated]

  • Gastrointestinal secretory protein
  • GISP
  • GISPREG-4
  • Reg IV
  • Reg4
  • regenerating gene type IV
  • regenerating islet-derived family, member 4
  • regenerating islet-derived protein 4
  • Regenerating islet-derived protein IV
  • REG-IV
  • RELP
  • RELPREG-like protein

Background

Reg4 (regenerating islet-derived protein 4; also named Reg-like protein, RELP, and gastrointestinal secretory protein) is a 17 kDa, secreted glycoprotein belonging to the calcium (C-type) dependent lectin superfamily and the regenerating gene family (1-3). There are three potential isoforms for human Reg4. Isoform 1 is synthesized as a 158 amino acid (aa) precursor with a 22 aa signal sequence and a 136 aa mature chain. Amino acids 30-156 constitute a C-type lectin-like domain, and amino acid 50 is a site for potential N-linked glycosylation. In addition, amino acids 127 and 142-143 form a ligand-binding surface. In isoform 2, there is a 79 aa substitution for the C-terminal 101 amino acids, while in isoform 3, there is an 11 aa substitution for the C-terminal 57 amino acids. Human Reg4 shares 68% and 67% aa sequence identity with rat and mouse Reg4, respectively. Like other members of the regenerating gene family, Reg4 is expressed in the gastrointestinal (GI) tract and ectopically at other sites in the setting of tissue injury (1, 2). In particular, Reg4 is expressed in normal colon mucosa, and up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, inflammatory bowel disease (Crohn’s disease and ulcerative colitis), and, outside the GI tract, in prostate adenocarcinoma (2-8). The physiological function of Reg4 is presently unknown, but it may be involved in inflammatory and metaplastic responses of the GI epithelium (3).

  1. Hartupee, J.C. et al. (2001) Biochim. Biophys. Acta 1518:287.
  2. Zhang, Y. et al. (2003) World J. Gastroenterol. 9:2635.
  3. Kamarainen, M. et al. (2003) Am. J. Pathol. 163:11.
  4. Zhang, Y. et al. (2003) Cancer Lett. 200:69.
  5. Violette, S. et al. (2003) Int. J. Cancer 103:185.
  6. Oue, N. et al. (2005) J. Pathol. 207:185.
  7. Zhennan, G. et al. (2005) Clin. Cancer Res. 2:2237
  8. Bishnupuri, K.S. et al. (2006) Gastroenterology 130:137.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Reg4 Antibody (AF1379)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-P, Western Blot.


Filter By Application
IHC
(2)
IHC-P
(2)
Western Blot
(2)
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Filter By Species
Human
(4)
Mouse
(2)
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Showing Publications 1 - 6 of 6.
Publications using AF1379 Applications Species
E Koren, Y Yosefzon, R Ankawa, D Soteriou, A Jacob, A Nevelsky, R Ben-Yosef, G Bar-Sela, Y Fuchs ARTS mediates apoptosis and regeneration of the intestinal stem cell niche Nat Commun, 2018;9(1):4582. 2018 [PMID: 30389919] (IHC, Mouse) IHC Mouse
M Ayars, E O'Sullivan, A Macgregor-, K Shindo, H Kim, M Borges, J Yu, RH Hruban, M Goggins IL2RG, identified as overexpressed by RNA-seq profiling of pancreatic intraepithelial neoplasia, mediates pancreatic cancer growth Oncotarget, 2017;8(48):83370-83383. 2017 [PMID: 29137350] (IHC-P, Human) IHC-P Human
Reg4+ deep crypt secretory cells function as epithelial niche for Lgr5+ stem cells in colon Proc Natl Acad Sci USA, 2016;113(37):E5399-407. 2016 [PMID: 27573849] (Western Blot, Mouse) Western Blot Mouse
Delker D, McGettigan B, Kanth P, Pop S, Neklason D, Bronner M, Burt R, Hagedorn C RNA sequencing of sessile serrated colon polyps identifies differentially expressed genes and immunohistochemical markers. PLoS ONE, 2014;9(2):e88367. 2014 [PMID: 24533081] (IHC, Human) IHC Human
Rafa L, Dessein AF, Devisme L, Buob D, Truant S, Porchet N, Huet G, Buisine MP, Lesuffleur T REG4 acts as a mitogenic, motility and pro-invasive factor for colon cancer cells. Int. J. Oncol., 2010;36(3):689-98. 2010 [PMID: 20126989] (Western Blot, Human) Western Blot Human
Mitani Y, Oue N, Matsumura S, Yoshida K, Noguchi T, Ito M, Tanaka S, Kuniyasu H, Kamata N, Yasui W Reg IV is a serum biomarker for gastric cancer patients and predicts response to 5-fluorouracil-based chemotherapy. Oncogene, 2007;26(30):4383-93. 2007 [PMID: 17237819] (IHC-P, Human) IHC-P Human

Review for Reg4 Antibody (AF1379) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF1379:
Filter by Applications
IHC-Fr
(1)
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Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
IHC-Fr Mouse 01/05/2015
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23769888
SpeciesMouse

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Biotin BAF1379

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Recent Reviews

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1
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Anonymous
01/05/2015
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol REG4
Uniprot