Claudin-1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Claudin-1 Antibody [NBP2-38578] - Staining in human skin and skeletal muscle tissues using NBP2-38578 antibody. Corresponding CLDN1 RNA-seq data are presented for the same tissues.

Western Blot: Claudin-1 Antibody [NBP2-38578] - Analysis in human cell lines A-431 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Immunohistochemistry-Paraffin: Claudin-1 Antibody [NBP2-38578] - Staining of human fallopian tube shows moderate to strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Claudin-1 Antibody [NBP2-38578] - Staining of human liver shows moderate membranous positivity in hepatocytes.

Immunohistochemistry-Paraffin: Claudin-1 Antibody [NBP2-38578] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: Claudin-1 Antibody [NBP2-38578] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Claudin-1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Claudin-1 Antibody - BSA Free (NBP2-38578) is a polyclonal antibody validated for use in IHC and WB. Anti-Claudin-1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: KTTSYPTPRPYPKPAPSSGKDYV
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLDN1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Claudin-1 Recombinant Protein Antigen (NBP2-38578PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Claudin-1 Antibody - BSA Free

• claudin 1
• Claudin1
• Claudin-1
• CLD1
• CLDN1
• ILVASCSenescence-associated epithelial membrane protein
• SEMP1
• SEMP1senescence-associated epithelial membrane protein 1

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claudin-1 Antibody (NBP2-38578)(1)

Publication using NBP2-38578

• Cheng Y, Li J, Zhang X et al. Protective Effect of Qingchang Wenzhong Decoction on Colitis and Colitis-Related Carcinogenesis by Regulating Inflammation and Intestinal Fibrosis Journal of inflammation research 2023-04-07 [PMID: 37056910] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: CLDN1
• Uniprot:
  • O95832()