Claspin Antibody [Unconjugated]

Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Claspin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3310) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Claspin at approximately 199 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Claspin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Claspin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3310) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Claspin Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Claspin
  Phe1138-Ser1339
  Accession # Q9HAW4
• Specificity: Detects human Claspin in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CLSPN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Claspin Antibody [Unconjugated]

• claspin homolog (Xenopus laevis)
• claspin homolog
• Claspin
• CLSPN
• hClaspin
• MGC131612
• MGC131613
• MGC131615

Background

Claspin is a nuclear protein whose expression peaks during S and G2 phases of the cell cycle. Claspin is phosphorylated by ATR in response to DNA damage or replication stress. It functions in cell cycle checkpoint control by regulating the activation of Chk1 and BRCA1. Claspin contains three CKB motifs and two coiled coil regions. Over the range used for immunization, human and mouse Claspin share 72% amino acid sequence identity. DNA replication and DNA damage induce the phosphorylation of Claspin by Chk1-dependent mechanisms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claspin Antibody (AF3310)(1)

Publication using AF3310

• J Wang, A Calizo, L Zhang, JC Pino, Y Lyu, K Pollard, X Zhang, AT Larsson, E Conniff, N Llosa, DK Wood, DA Largaespad, SE Moody, SJ Gosline, AC Hirbe, CA Pratilas CDK4/6 inhibition enhances SHP2 inhibitor efficacy and is dependent upon restoration of RB function in malignant peripheral nerve sheath tumors bioRxiv : the preprint server for biology, 2023-02-03;0(0):. 2023-02-03 [PMID: 36778419] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CLSPN
• Uniprot:
  • Q9HAW4()