CKAP4/p63 Antibody

Western Blot: CKAP4/p63 Antibody [NBP1-26643] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. CKAP4 was also immunoprecipitated by rabbit anti-CKAP4 antibodies NBP1-26641 and NBP1-26642.

• Reactivity: Hu, Mu, Ma
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CKAP4/p63 Antibody Summary

• Description: Novus Biologicals Rabbit CKAP4/p63 Antibody (NBP1-26643) is a polyclonal antibody validated for use in WB and IP. Anti-CKAP4/p63 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 552 and 602 of human cytoskeleton-associated protein 4 using the numbering given in entry NP_006816.2 (GeneID 10970).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CKAP4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 3-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID: 27180817).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CKAP4/p63 Antibody

• 63 kDa membrane protein
• CKAP4
• CLIMP-63
• cytoskeleton-associated protein 4
• ERGIC-63
• MGC99554
• p63
• transmembrane protein (63kD), endoplasmic reticulum/Golgi intermediatecompartment
• type-II transmembrane protein p63

Background

Cytoskeleton-associated protein 4 (CKAP4) is also known as p63 and cytoskeleton-linking membrane protein 63kDa (CLIMP-63). It was first described as a palmitoylated single-pass transmembrane receptor that localized to the rough endoplasmic reticulum as well as the cell membrane. CKAP4 has been shown to help anchor endoplasmic reticulum to microtubules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CKAP4/p63 Antibody (NBP1-26643)(2)

Publications using NBP1-26643

• Song JW, Zhu J, Wu XX Et al. GOLPH3/CKAP4 promotes metastasis and tumorigenicity by enhancing the secretion of exosomal WNT3A in non-small-cell lung cancer Cell death & disease 2021-10-21 [PMID: 34671013] (PLA, Human)
• Mandal A, Mandal S, Park MH. Global quantitative proteomics reveal up-regulation of endoplasmic reticulum stress response proteins upon depletion of eIF5A in HeLa cells. Nature Publishing Group. 2016-05-16 [PMID: 27180817] (WB, Mammal)

Review for CKAP4/p63 Antibody (NBP1-26643) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/16/2015

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: CKAP4
• Entrez:
  • 10970(Human)
  • 216197(Mouse)
• Uniprot:
  • Q07065(Human)