CKAP4/p63 Antibody

Western Blot: CKAP4/p63 Antibody [NBP1-26642] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. CKAP4 was also immunoprecipitated by rabbit anti-CKAP4 antibodies NBP1-26641and NBP1-26643.

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-26642] - Human breast carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CKAP4/p63 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 500 and 550 of human cytoskeleton-associated protein 4 using the numbering given in entry NP_006816.2 (GeneID 10970).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CKAP4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200- 1:1000
  • Immunohistochemistry-Paraffin 1:200- 1:1000
  • Immunoprecipitation 3-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CKAP4/p63 Antibody

• 63 kDa membrane protein
• CKAP4
• CLIMP-63
• cytoskeleton-associated protein 4
• ERGIC-63
• MGC99554
• p63
• transmembrane protein (63kD), endoplasmic reticulum/Golgi intermediatecompartment
• type-II transmembrane protein p63

Background

Cytoskeleton-associated protein 4 (CKAP4) is also known as p63 and cytoskeleton-linking membrane protein 63kDa (CLIMP-63). It was first described as a palmitoylated single-pass transmembrane receptor that localized to the rough endoplasmic reticulum as well as the cell membrane. CKAP4 has been shown to help anchor endoplasmic reticulum to microtubules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CKAP4/p63 Antibody (NBP1-26642)(1)

Publication using NBP1-26642

• Agemy L, Kotamraju VR, Friedmann-Morvinski D et al. Proapoptotic Peptide-Mediated Cancer Therapy Targeted to Cell Surface p32. Mol Ther. 2013-08-20 [PMID: 23959073] (WB, Human, Mouse)

Bioinformatics

• Gene Symbol: CKAP4
• Entrez:
  • 10970(Human)
• Uniprot:
  • Q07065(Human)