CITED4 Antibody (HT13-2D6.3)

Images

 
Western Blot: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - Detection of human CITED4 in 20ug of transfected whole cell Hep3B lysate using NB110-415752. Photo courtesy of Dr. DeCaprio, DFCI.
Immunohistochemistry-Paraffin: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - CITED4 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human CITED4 (HT13-2D6.3) Monoclonal Antibody ...read more
Flow Cytometry: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using CITED4 antibody (dark blue). Isotype control shown in orange. An ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Clone
HT13-2D6.3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CITED4 Antibody (HT13-2D6.3) Summary

Immunogen
N-terminal fusion protein to full-length human CITED4 [UniProt# Q96RK1]
Localization
Cytoplasmic and Nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CITED4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
This CITED4 antibody is useful for ELISA, Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Flow Cytometry and Western blot using CITED4 transfected cells. Immunohistochemistry-Paraffin was reported in scientific literature.In Western blot a band was observed ~25 kDa. A lower (15 kDa) non-specific band may also be seen. Dilutions should be determined by the researcher.
Publications
Read Publication using
NB110-41572 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CITED4 Antibody (HT13-2D6.3)

  • cbp/p300-interacting transactivator 4
  • Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain4,MRG-2
  • MRG2
  • MSG1-related protein 2
  • transcriptional co-activator 4

Background

CITED4 (CREB-binding protein/p300-interacting transactivator with E/D-rich tail 4) belongs to CITED family and all members of this family share CITED domain that can interact with CBP and p300. These proteins are transcriptional co-activators which can increase gene transcription through binding transcription factors with RNA polymerase II or via their acetyltransferase activity. CITED4 behaves as transcriptional coactivator for TFAP2/AP-2 and enhances estrogen receptors mediated transactivation. It can disrupt HIF1A -CREBBP interaction for acting as inhibitor of HIF1A mediated transactivation. CITED4 implicates in gene expression regulation during development/differentiation of blood cells, endothelial cells as well as mammary epithelial cells. CITED4 primarily localize to nucleus, however, its cytoplasmatic translocation or loss of nuclear expression has been observed in breast cancer where it is considered a prognostic marker for carcinogenesis. Moreover, in breast cancer its expression is down-regulated and often correlate with elevated HIF1A levsls, tumor growth and angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CITED4 Antibody (NB110-41572)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA, IHC-P, IP, WB.


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ELISA
(1)
IHC-P
(1)
IP
(1)
WB
(1)
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Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CITED4