CITED4 Antibody (HT13-2D6.3) - BSA Free

Western Blot: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - Detection of human CITED4 in 20ug of transfected whole cell Hep3B lysate using NB110-415752. Photo courtesy of Dr. DeCaprio, DFCI.

Immunohistochemistry-Paraffin: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - CITED4 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human CITED4 (HT13-2D6.3) Monoclonal Antibody (Catalog # NB110-41572) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in cardiomyocytes.

Flow Cytometry: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using CITED4 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP
• Clone: HT13-2D6.3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CITED4 Antibody (HT13-2D6.3) - BSA Free Summary

• Immunogen: N-terminal fusion protein to full-length human CITED4 [UniProt# Q96RK1]
• Localization: Cytoplasmic and Nuclear
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CITED4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin reported in scientific literature
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This CITED4 antibody is useful for CITED4 transfected cells. In Western blot a band was observed ~25 kDa. A lower (15 kDa) non-specific band may also be seen. Dilutions should be determined by the researcher.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CITED4 Antibody (HT13-2D6.3) - BSA Free

• cbp/p300-interacting transactivator 4
• Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain4,MRG-2
• MRG2
• MSG1-related protein 2
• transcriptional co-activator 4

Background

CITED4 (CREB-binding protein/p300-interacting transactivator with E/D-rich tail 4) belongs to CITED family and all members of this family share CITED domain that can interact with CBP and p300. These proteins are transcriptional co-activators which can increase gene transcription through binding transcription factors with RNA polymerase II or via their acetyltransferase activity. CITED4 behaves as transcriptional coactivator for TFAP2/AP-2 and enhances estrogen receptors mediated transactivation. It can disrupt HIF1A -CREBBP interaction for acting as inhibitor of HIF1A mediated transactivation. CITED4 implicates in gene expression regulation during development/differentiation of blood cells, endothelial cells as well as mammary epithelial cells. CITED4 primarily localize to nucleus, however, its cytoplasmatic translocation or loss of nuclear expression has been observed in breast cancer where it is considered a prognostic marker for carcinogenesis. Moreover, in breast cancer its expression is down-regulated and often correlate with elevated HIF1A levsls, tumor growth and angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CITED4 Antibody (NB110-41572)(1)

Publication using NB110-41572

• Fox, SB et al. CITED4 inhibits hypoxia-activated transcription in cancer cells, and its cytoplasmic location in breast cancer is associated with elevated expression of tumor cell hypoxia-inducible factor 1alpha. Cancer Res;64(17):6075-81. 2004-09-01 [PMID: 15342390] (IP, ELISA, WB, IHC-P, Human)

Bioinformatics

• Gene Symbol: CITED4