CITED4 Antibody (HT13-2D6.3) [FITC]

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP
• Clone: HT13-2D6.3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

CITED4 Antibody (HT13-2D6.3) [FITC] Summary

• Immunogen: N-terminal fusion protein to full-length human CITED4 [UniProt# Q96RK1]
• Localization: Cytoplasmic and Nuclear
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CITED4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for CITED4 Antibody (HT13-2D6.3) [FITC]

• cbp/p300-interacting transactivator 4
• Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain4,MRG-2
• MRG2
• MSG1-related protein 2
• transcriptional co-activator 4

Background

CITED4 (CREB-binding protein/p300-interacting transactivator with E/D-rich tail 4) belongs to CITED family and all members of this family share CITED domain that can interact with CBP and p300. These proteins are transcriptional co-activators which can increase gene transcription through binding transcription factors with RNA polymerase II or via their acetyltransferase activity. CITED4 behaves as transcriptional coactivator for TFAP2/AP-2 and enhances estrogen receptors mediated transactivation. It can disrupt HIF1A -CREBBP interaction for acting as inhibitor of HIF1A mediated transactivation. CITED4 implicates in gene expression regulation during development/differentiation of blood cells, endothelial cells as well as mammary epithelial cells. CITED4 primarily localize to nucleus, however, its cytoplasmatic translocation or loss of nuclear expression has been observed in breast cancer where it is considered a prognostic marker for carcinogenesis. Moreover, in breast cancer its expression is down-regulated and often correlate with elevated HIF1A levsls, tumor growth and angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CITED4