CITED4 Antibody (HT13-2D6.3) [FITC]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP
Clone
HT13-2D6.3
Clonality
Monoclonal
Host
Mouse

CITED4 Antibody (HT13-2D6.3) [FITC] Summary

Immunogen
N-terminal fusion protein to full-length human CITED4 [UniProt# Q96RK1]
Localization
Cytoplasmic and Nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CITED4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
FITC
Format
FITC (A=495, E=519)
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
This CITED4 antibody is useful for ELISA, Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Western blot using CITED4 transfected cells. Immunohistochemistry-Paraffin was reported in scientific literature.In Western blot a band was observed ~25 kDa. A lower (15 kDa) non-specific band may also be seen. Dilutions should be determined by the researcher.

Reactivity Notes

Human.

Alternate Names for CITED4 Antibody (HT13-2D6.3) [FITC]

  • cbp/p300-interacting transactivator 4
  • Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain4,MRG-2
  • MRG2
  • MSG1-related protein 2
  • transcriptional co-activator 4

Background

CITED4 (CREB-binding protein/p300-interacting transactivator with E/D-rich tail 4) belongs to CITED family and all members of this family share CITED domain that can interact with CBP and p300. These proteins are transcriptional co-activators which can increase gene transcription through binding transcription factors with RNA polymerase II or via their acetyltransferase activity. CITED4 behaves as transcriptional coactivator for TFAP2/AP-2 and enhances estrogen receptors mediated transactivation. It can disrupt HIF1A -CREBBP interaction for acting as inhibitor of HIF1A mediated transactivation. CITED4 implicates in gene expression regulation during development/differentiation of blood cells, endothelial cells as well as mammary epithelial cells. CITED4 primarily localize to nucleus, however, its cytoplasmatic translocation or loss of nuclear expression has been observed in breast cancer where it is considered a prognostic marker for carcinogenesis. Moreover, in breast cancer its expression is down-regulated and often correlate with elevated HIF1A levsls, tumor growth and angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CITED4 Antibody (NB110-41572F) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB110-41572APC
Biotin Labeled NB110-41572B
DyLight 350 Labeled NB110-41572UV
DyLight 405 Labeled NB110-41572V
DyLight 405LS Labeled NB110-41572V3
DyLight 488 Labeled NB110-41572G
DyLight 550 Labeled NB110-41572R
DyLight 650 Labeled NB110-41572C
DyLight 680 Labeled NB110-41572FR
DyLight 755 Labeled NB110-41572IR
HRP Labeled NB110-41572H
PE Labeled NB110-41572PE
PerCP Labeled NB110-41572PCP

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Blogs on CITED4

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol CITED4

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