CIP2A Antibody (2G10)

Images

 
Western Blot: CIP2A Antibody (2G10) [NB110-59722] - Analysis of CIP2A in NIH/3T3 cell lysate.
Immunocytochemistry/ Immunofluorescence: CIP2A Antibody (2G10) [NB110-59722] - CIP2A antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Immunohistochemistry-Paraffin: CIP2A Antibody (2G10) [NB110-59722] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using CIP2A antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). ...read more
Flow Cytometry: CIP2A Antibody (2G10) [NB110-59722] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using CIP2A antibody (dark blue). Isotype control shown in orange. An antibody ...read more
Western Blot: CIP2A Antibody (2G10) [NB110-59722] - Detection of CIP2A in HeLa whole cell lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
2G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.8 mg/ml

CIP2A Antibody (2G10) Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human CIP2A protein. [UniProt# Q8TCG1]
Epitope
A 300 amino acid portion of the C-terminus.
Localization
Cytoplasmic
Specificity
p90 autoantigen (2G10)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KIAA1524
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:40
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200
Application Notes
This CIP2A antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot. In ICC/IF, strong cytoplasmic staining is observed in U20s cells. In Western Blot analysis, a band is seen at ~90 kDa representing CIP2A. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 25965834)
Positive Control
HeLa Lysate (NB800-PC1)
CIP2A Lysate (NBL1-12266)
Publications
Read Publications using
NB110-59722 in the following applications:

Reactivity Notes

Human, mouse, rat and primate. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.8 mg/ml
Purity
Protein G purified

Alternate Names for CIP2A Antibody (2G10)

  • Cancerous inhibitor of PP2A
  • CIP2A
  • FLJ12850
  • KIAA1524
  • MGC163436
  • p90 autoantigen
  • p90
  • protein CIP2A

Background

Inhibition of protein phosphatase 2A (PP2A) activity has been identified as a prerequisite for the transformation of human cells. The protein, designated Cancerous Inhibitor of PP2A (CIP2A), interacts directly with the oncogenic transcription factor c-Myc, inhibits PP2A activity toward c-Myc serine 62 (S62), and thereby prevents c-Myc proteolytic degradation. In addition to its function in c-Myc stabilization, CIP2A promotes anchorage-independent cell growth and in vivo tumor formation. The oncogenic activity of CIP2A is demonstrated by transformation of human cells by overexpression of CIP2A. Importantly, CIP2A is overexpressed in two common human malignancies, head and neck squamous cell carcinoma (HNSCC) and colon cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CIP2A Antibody (NB110-59722)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(4)
IHC-P
(1)
WB
(9)
All Applications
Filter By Species
Human
(10)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB110-59722 Applications Species
Zhao S, Gao X, Zang S et al. MicroRNA-383-5p acts as a prognostic marker and inhibitor of cell proliferation in lung adenocarcinoma by cancerous inhibitor of protein phosphatase 2A Oncology Letters 2017 Jul 18 [PMID: 28927114] (WB, Human) WB Human
Wang X, Gao P, Wang M et al. Feedback between E2F1 and CIP2A regulated by human papillomavirus E7 in cervical cancer: implications for prognosis.. Am J Transl Res May 31 2017 [PMID: 28559983] (WB, ICC/IF, IHC, Human) WB, ICC/IF, IHC Human
Khanna A, Rane JK, Kivinummi KK et al. CIP2A is a candidate therapeutic target in clinically challenging prostate cancer cell populations. Oncotarget. 2015 Apr 19 [PMID: 25965834] (IHC-P, Human) IHC-P Human
Jung HM, Phillips BL, Chan EK. miR-375 activates p21 and suppresses telomerase activity by coordinately regulating HPV E6/E7, E6AP, CIP2A, and 14-3-3zeta. Mol. Cancer 2014 Apr 17 [PMID: 24708873] (WB, Human) WB Human
Zhai M, Cong L, Han Y et al. CIP2A is overexpressed in osteosarcoma and regulates cell proliferation and invasion. Tumour Biol. 2013 Sep 8 [PMID: 24014087]
Jung HM, Patel RS, Phillips BL et al. Tumor suppressor miR-375 regulates MYC expression via repression of CIP2A coding sequence through multiple miRNA-mRNA interactions. Mol Biol Cell 2013 Jun [PMID: 23552692] (WB, Human) WB Human
Lee J, Jeong H, Park EJ et al. CIP2A facilitates apoptotic resistance of fibroblast-like synoviocytes in rheumatoid arthritis independent of c-Myc expression. Rheumatol Int 2013 Mar 2 [PMID: 23455633] (WB, Human) WB Human
Ma L, Wen Z-S, Liu Z et al. Overexpression and Small Molecule-Triggered Downregulation of CIP2A in Lung Cancer. PLoS One;6(5):e20159. 2011 [PMID: 21655278] (WB, IHC, Human) WB, IHC Human
Lee J, Park EJ, Hwang JW et al. CIP2A expression is associated with synovial hyperplasia and invasive function of fibroblast-like synoviocytes in rheumatoid arthritis Rheumatol Int 2012 Jul [PMID: 21479604] (WB, IHC, Human) WB, IHC Human
Wang J, Li W, Li L, Yu X, Jia J, Chen C. CIP2A is over-expressed in acute myeloid leukaemia and associated with HL60 cells proliferation and differentiation. Int J Lab Hematol;33(3):290-8.doi: 10.1111/j.1751-553X.2010.01288.x. 2011 Jun. [PMID: 21219591] (WB, Human) WB Human
Show All 11 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CIP2A Antibody (NB110-59722) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-59722AF405
Alexa Fluor 488 Labeled NB110-59722AF488
Alexa Fluor 647 Labeled NB110-59722AF647
Alexa Fluor 700 Labeled NB110-59722AF700
Allophycocyanin Labeled NB110-59722APC
Biotin Labeled NB110-59722B
DyLight 350 Labeled NB110-59722UV
DyLight 405 Labeled NB110-59722V
DyLight 488 Labeled NB110-59722G
DyLight 550 Labeled NB110-59722R
DyLight 650 Labeled NB110-59722C
DyLight 680 Labeled NB110-59722FR
DyLight 755 Labeled NB110-59722IR
FITC Labeled NB110-59722F
HRP Labeled NB110-59722H
PE Labeled NB110-59722PE

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Blogs on CIP2A.

CIP2A - A lean, mean, oncoprotein machine
The CIP2A protein was originally identified as p90, a cytoplasmic auto-antigen from the serum of a cancer patient. It was later found to inhibit protein phosphatase 2A (PP2A) activity as well as interact with c-myc. CIP2A's inhibitory activity blocks...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2A....  Read full blog post.

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Bioinformatics

Gene Symbol KIAA1524