CIP2A Antibody (2G10) - Azide and BSA Free

Western Blot: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670] - Detection of CIP2A in HeLa whole cell lysate. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670] - CIP2A antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using CIP2A antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (

Flow Cytometry: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using CIP2A antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.

Western Blot: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670] - Analysis of CIP2A in NIH/3T3 cell lysate. Image from the standard format of this antibody.

Western Blot: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670] - miR-375 increases p21, p53, and RB in HPV16- and 18-positive cancer. Protein levels of CIP2A, p53, and p21 in SiHa cells transfected with si-CIP2A and/or si-p53 were measured by Western blot analysis. Protein levels of CIP2A, p53, and p21 in SiHa cells transfected with si-p53 and/or miR-375-mimic were measured by Western blot analysis. Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-80) licensed under a CC-BY license. Image from the standard format of this antibody.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, ICC/IF, IHC, Mycoplasma
• Clone: 2G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

CIP2A Antibody (2G10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CIP2A Antibody (2G10) - Azide and BSA Free (NB110-59722) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to a C-terminal portion of the human CIP2A protein. [UniProt# Q8TCG1]
• Epitope: A 300 amino acid portion of the C-terminus.
• Localization: Cytoplasmic
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CIP2A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence 1:40
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 25965834)
  • Knockdown Validated
  • Western Blot 1:1000-1:2000
• Application Notes: In ICC/IF, strong cytoplasmic staining is observed in U20s cells. In Western Blot analysis, a band is seen at ~90 kDa representing CIP2A.

Reactivity Notes

Other species have not been tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CIP2A Antibody (2G10) - Azide and BSA Free

• Cancerous inhibitor of PP2A
• CIP2A
• FLJ12850
• KIAA1524
• MGC163436
• p90 autoantigen
• p90
• protein CIP2A

Background

Inhibition of protein phosphatase 2A (PP2A) activity has been identified as a prerequisite for the transformation of human cells. The protein, designated Cancerous Inhibitor of PP2A (CIP2A), interacts directly with the oncogenic transcription factor c-Myc, inhibits PP2A activity toward c-Myc serine 62 (S62), and thereby prevents c-Myc proteolytic degradation. In addition to its function in c-Myc stabilization, CIP2A promotes anchorage-independent cell growth and in vivo tumor formation. The oncogenic activity of CIP2A is demonstrated by transformation of human cells by overexpression of CIP2A. Importantly, CIP2A is overexpressed in two common human malignancies, head and neck squamous cell carcinoma (HNSCC) and colon cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CIP2A