cIAP-1/HIAP-2 Antibody - BSA Free

Western Blot: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Western Blot Validation in Human and Rat Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: cIAP NBP1-77193 (1 ug/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Analysis in human lung lysate with antibody at 1 (lane A), 2 (lane B), and 4 (lane C) ug/mL, respectively.

Western Blot: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: cIAP NBP1-77193 (2 ug/mL), cIAP, competitor antibody, (1 ug/mL) and beta-actin NBP1-76692 (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: cIAP NBP1-77193 (2 ug/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/Immunofluorescence: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Human Lung cells with cIAP-1/HIAP-2 antibody at 20ug/ml

Immunohistochemistry: cIAP-1/HIAP-2 Antibody - BSA Free [NBP1-77193] - Validation of cIAP-1/HIAP-2 in Human Lung Tissue. Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-cIAP-1/HIAP-2 antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• Reactivity: Hu, Mu, Rt, Po, Ch
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

cIAP-1/HIAP-2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit cIAP-1/HIAP-2 Antibody - BSA Free (NBP1-77193) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human cIAP-1/HIAP-2. c-IAP antibody detects both c-IAP1 and c-IAP2. The immunogen is located within amino acids 540-590 of cIAP-1/HIAP-2. Amino Acid Sequence: SLEEQLRRLQEERT
• Specificity: Human cIAP-1/HIAP-2 has 2 isoforms, including isoform 1 (618aa, 70kD) and isoform 2 (569aa, 64kD). Mouse cIAP-1/HIAP-2 has one isoform (612aa, 70kD) and Rat cIAP-1/HIAP-2 also has one isoform (589aa, 67kD). NBP1-77193 can detect human, mouse and rat.
• Predicted Species: Porcine (93%), Chicken (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BIRC2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 10 ug/mL
  • Immunohistochemistry-Paraffin
  • Western Blot 1-4 ug/ml
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  cIAP-1/HIAP-2 Peptide (NBP1-77193PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for cIAP-1/HIAP-2 Antibody - BSA Free

• API1Hiap-2
• apoptosis inhibitor 1
• baculoviral IAP repeat containing 2
• baculoviral IAP repeat-containing 2
• baculoviral IAP repeat-containing protein 2
• BIRC2
• cIAP1
• c-IAP1
• cIAP-1
• hIAP2
• HIAP-2
• IAP homolog B
• IAP2
• IAP-2
• Inhibitor of apoptosis protein 2
• MIHB
• MIHBHIAP2
• NFR2-TRAF signalling complex protein
• RING finger protein 48
• RNF48hiap-2
• TNFR2-TRAF-signaling complex protein 2

Background

Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The two isoforms of c-IAP (c-IAP1 and c-IAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7. The c-IAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF-alpha by its receptor. Omi, a negative regulator of c-IAP, inhibits its activity by catalytically cleaving c-IAP. Another negative regulator, Smac/DIABLO, acts by enhancing the auto-ubiquitization activity of c-IAP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BIRC2
• Entrez:
  • 329(Human)
  • 11797(Mouse)
• Uniprot:
  • Q13490()