cIAP-1/HIAP-2 Antibody (HL1045) - Azide and BSA Free

Western Blot: cIAP-1/HIAP-2 Antibody (HL1045) [NBP3-13700] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP-1/HIAP-2 antibody [HL1045] (NBP3-13700) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: cIAP-1/HIAP-2 Antibody (HL1045) [NBP3-13700] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP-1/HIAP-2 antibody [HL1045] (NBP3-13700) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: cIAP-1/HIAP-2 Antibody (HL1045) [NBP3-13700] - cIAP-1/HIAP-2 antibody [HL1045] detects cIAP-1/HIAP-2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog pancreas.cIAP-1/HIAP-2 stained by cIAP-1/HIAP-2 antibody [HL1045] (NBP3-13700) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: cIAP-1/HIAP-2 Antibody (HL1045) [NBP3-13700] - cIAP-1/HIAP-2 antibody [HL1045] detects cIAP-1/HIAP-2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat lung.cIAP-1/HIAP-2 stained by cIAP-1/HIAP-2 antibody [HL1045] (NBP3-13700) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: cIAP-1/HIAP-2 Antibody (HL1045) [NBP3-13700] - cIAP-1/HIAP-2 antibody [HL1045] detects cIAP-1/HIAP-2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat spleen.cIAP-1/HIAP-2 stained by cIAP-1/HIAP-2 antibody [HL1045] (NBP3-13700) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: cIAP-1/HIAP-2 Antibody (HL1045) [NBP3-13700] - cIAP-1/HIAP-2 antibody [HL1045] detects cIAP-1/HIAP-2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.cIAP-1/HIAP-2 stained by cIAP-1/HIAP-2 antibody [HL1045] (NBP3-13700) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: cIAP-1/HIAP-2 Antibody (HL1045) [NBP3-13700] - Non-transfected (–) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody [HL1045] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB
• Clone: HL1045
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

cIAP-1/HIAP-2 Antibody (HL1045) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the center region of human cIAP-1/HIAP-2. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BIRC2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for cIAP-1/HIAP-2 Antibody (HL1045) - Azide and BSA Free

• API1Hiap-2
• apoptosis inhibitor 1
• baculoviral IAP repeat containing 2
• baculoviral IAP repeat-containing 2
• baculoviral IAP repeat-containing protein 2
• BIRC2
• cIAP1
• C-IAP1
• cIAP-1
• hIAP2
• HIAP-2
• IAP homolog B
• IAP2
• IAP-2
• Inhibitor of apoptosis protein 2
• MIHB
• MIHBHIAP2
• NFR2-TRAF signalling complex protein
• RING finger protein 48
• RNF48hiap-2
• TNFR2-TRAF-signaling complex protein 2

Background

cIAP1 (HIAP2, MIHB) is a member of the family of inhibitor of apoptosis proteins (IAP). IAPs suppress mitochondria-dependent and -independent apoptosis by binding to and inhibiting caspases through their BIR domains (reviewed in Liston et al, 2003; Wright and Duckett, 2005). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis. In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal. The RING finger domain of several IAPs, including cIAP2, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubqiuitination. Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival. The IAPs, including cIAP1, have widespread tissue protein expression, with expression levels and subcellular localization patterns differing depending on the cell lineage (see Vischioni et al. 2005 for a comprehensive study). This antibody recognizes cIAP1, human cIAP1 is a 618 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BIRC2