Chromogranin A Antibody (LK2H10 + PHE5)

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5) [NBP2-34239] - Formalin-fixed paraffin-embedded human Adrenal Gland stained with Chromogranin A Monoclonal Antibody (PHE5)

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5) [NBP2-34239] - Formalin-fixed, paraffin-embedded human small cell lung carcinoma stained with Chromogranin A MAb (LK2H10+PHE5)

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: LK2H10 + PHE5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Chromogranin A Antibody (LK2H10 + PHE5) Summary

• Immunogen: Human pheochromocytoma (LK2H10 & PHE5)
• Localization: Finely granular cytoplasmic
• Marker: Neuroendocrine Marker
• Specificity: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
• Isotype: IgG1/IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHGA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug protein lysate
• Application Notes: Human Chromosome Location: 14q32.12
  Mol. Weight of Antigen: 68-75kDa
  Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for Chromogranin A Antibody (LK2H10 + PHE5)

• betagranin (N-terminal fragment of chromogranin A)
• Betagranin
• Catestatin
• CGA
• CHGA
• Chromofungin
• chromogranin A (parathyroid secretory protein 1)
• Chromogranin A
• chromogranin-A
• Pancreastatin
• Parastatin
• Parathyroid Secretory Protein 1
• Pituitary Secretory Protein I
• SP-I

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Chromogranin A Antibody (NBP2-34239). (Showing 1 - 1 of 1 FAQ).

1. I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?
   • When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.

Bioinformatics

• Gene Symbol: CHGA
• Uniprot:
  • P10645()