CHREBP Antibody (2D9NB) - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: CHREBP Antibody (2D9NB) [NBP2-44307] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells ...read more
Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) [NBP2-44307] - Analysis of a FFPE tissue section of human hepatocellular carcinoma using 1:200 dilution of CHREBP antibody (clone 2D9NB). The antibody generated a ...read more
Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) [NBP2-44307] - Analysis of a FFPE tissue section of normal human kidney using 1:200 dilution of CHREBP antibody (clone 2D9NB). The antibody generated a diffused to ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, ChIP
Clone
2D9NB
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

CHREBP Antibody (2D9NB) - BSA Free Summary

Immunogen
Partial recombinant human ChREBP protein between amino acids 600-800. [Uniprot: Q9NP71]
Localization
Cytoplasm, Nucleus
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MLXIPL
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:500 ug/ml
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31289120).
Publications
Read Publications using
NBP2-44307 in the following applications:

  • 1 publication
  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31289120). Use in Rat reported in scientific literature (PMID:19363290).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CHREBP Antibody (2D9NB) - BSA Free

  • BHLHD14
  • CHREBPcarbohydrate-responsive element-binding protein
  • Class D basic helix-loop-helix protein 14
  • MIOWS basic-helix-loop-helix leucine zipper protein
  • MLX interacting protein-like
  • MLX interactor
  • MLX-interacting protein-like
  • MONDOBWilliams-Beuren syndrome chromosomal region 14 protein
  • WBSCR14Williams-Beuren syndrome chromosome region 14 protein 1
  • Williams Beuren syndrome chromosome region 14
  • Williams-Beuren syndrome chromosome region 14 protein 2
  • WS-bHLHbHLHd14carbohydrate response element binding protein

Background

Carbohydrate response element-binding protein (ChREBP) is a glucose sensitive basic helix-loop-helix/leucine zipper transcription factor which heterodimerise with MLX and binds to carbohydrate response element (ChoRE) on DNA for the induction genes involved in glycolysis, de novo lipogenesis (DNL), and fatty acid desaturation. Some of the target genes of ChREBP are glucokinase (GCK), fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), pyruvate kinase/liver type pyruvate kinase (PK1/ PKLR), delta-9-desaturase (SCD/SCD1) etc. ChREBP is expressed as alpha and beta alternatively spliced isoforms, and among these two isoforms, the alpha-isoform is activated in a glucose-dependent manner. ChREBP is expressed in the cytoplasm and nuclei of a number of mammalian tissues, with high levels found in liver, skeletal muscle, white/brown adipose, heart, kidney, cerebellum and intestine. Glucose stimulates ChREBP transcriptional expression and activates the transactivity of ChREBP protein. It is activated independent of insulin and recent findings have suggested that together with its binding partner Mlx, ChREBP implicates in metabolic processes, insulin signaling related pathways, and also tumorigenesis/cancers. Also, increased hepatic ChREBP expression has been suggested to result in development of non-alcoholic fatty liver disease (NAFLD) and obesity through the conversion of carbohydrates into triglycerides (de novo lipogenesis).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CHREBP Antibody (NBP2-44307)(4)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CHREBP Antibody (NBP2-44307) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on CHREBP.

Deficiency of GluT1 leads to neurological problems while excess is involved in cancers
By Jamshed Arslan, Pharm. D., PhD. What Are GluTs?Mammalian cell metabolism is incomplete without glucose   . Glucose is a monosaccharide that is transported to the cells through facilitative diffusion, a ...  Read full blog post.

Insulin signaling in adipocytes: Carbohydrate-signaling transcription factor ChREBP is the link between lipolytic enzyme Hormone-Sensitive Lipase and lipogenic enzyme ELOVL6
By Jamshed Arslan, Pharm. D., PhD. Insulin resistance in adipocytes is a major feature of metabolic syndrome   . Disrupted adipose tissue metabolism can lead to accumulation of lipid intermediates in insul...  Read full blog post.

ChREBP, a glucose sensitive transcription factor with role in glucose-lipids homeostasis and cancer
ChREBP (carbohydrate response element-binding protein) is a glucose responsive basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor that binds MLX and then carbohydrate response element /ChoRE for the induction of genes involved in ...  Read full blog post.

ChREBP and Fatty Acid Biosynthesis Regulation
Carbohydrate response element binding protein (ChREBP) is a transcription factor involved in activating genes that encode enzymes of fatty acid biosynthesis in liver and adipose. It is believed to be a key controller of hepatic lipogenesis and has a p...  Read full blog post.

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Bioinformatics

Gene Symbol MLXIPL
Entrez
Uniprot