CHREBP Antibody (2D9NB)

Images

 
Immunocytochemistry/ Immunofluorescence: CHREBP Antibody (2D9NB) [NBP2-44307] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells ...read more
Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) [NBP2-44307] - Analysis of a FFPE tissue section of human hepatocellular carcinoma using 1:200 dilution of CHREBP antibody (clone 2D9NB). The antibody generated a ...read more
Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) [NBP2-44307] - Analysis of a FFPE tissue section of normal human kidney using 1:200 dilution of CHREBP antibody (clone 2D9NB). The antibody generated a diffused to ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, ChIP
Clone
2D9NB
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CHREBP Antibody (2D9NB) Summary

Immunogen
Partial recombinant human ChREBP protein between amino acids 600-800. [Uniprot: Q9NP71]
Localization
Cytoplasm, Nucleus
Predicted Species
Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MLXIPL
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Chromatin Immunoprecipitation (ChIP)
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31289120).
Publications
Read Publications using
NBP2-44307 in the following applications:

  • 1 publication
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31289120).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CHREBP Antibody (2D9NB)

  • BHLHD14
  • CHREBPcarbohydrate-responsive element-binding protein
  • Class D basic helix-loop-helix protein 14
  • MIOWS basic-helix-loop-helix leucine zipper protein
  • MLX interacting protein-like
  • MLX interactor
  • MLX-interacting protein-like
  • MONDOBWilliams-Beuren syndrome chromosomal region 14 protein
  • WBSCR14Williams-Beuren syndrome chromosome region 14 protein 1
  • Williams Beuren syndrome chromosome region 14
  • Williams-Beuren syndrome chromosome region 14 protein 2
  • WS-bHLHbHLHd14carbohydrate response element binding protein

Background

Carbohydrate response element-binding protein (ChREBP) is a glucose sensitive basic helix-loop-helix/leucine zipper transcription factor which heterodimerise with MLX and binds to carbohydrate response element (ChoRE) on DNA for the induction genes involved in glycolysis, de novo lipogenesis (DNL), and fatty acid desaturation. Some of the target genes of ChREBP are glucokinase (GCK), fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), pyruvate kinase/liver type pyruvate kinase (PK1/ PKLR), delta-9-desaturase (SCD/SCD1) etc. ChREBP is expressed as alpha and beta alternatively spliced isoforms, and among these two isoforms, the alpha-isoform is activated in a glucose-dependent manner. ChREBP is expressed in the cytoplasm and nuclei of a number of mammalian tissues, with high levels found in liver, skeletal muscle, white/brown adipose, heart, kidney, cerebellum and intestine. Glucose stimulates ChREBP transcriptional expression and activates the transactivity of ChREBP protein. It is activated independent of insulin and recent findings have suggested that together with its binding partner Mlx, ChREBP implicates in metabolic processes, insulin signaling related pathways, and also tumorigenesis/cancers. Also, increased hepatic ChREBP expression has been suggested to result in development of non-alcoholic fatty liver disease (NAFLD) and obesity through the conversion of carbohydrates into triglycerides (de novo lipogenesis).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CHREBP Antibody (NBP2-44307)(3)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CHREBP Antibody (NBP2-44307) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-44307AF350
Alexa Fluor 405 NBP2-44307AF405
Alexa Fluor 488 NBP2-44307AF488
Alexa Fluor 532 NBP2-44307AF532
Alexa Fluor 594 NBP2-44307AF594
Alexa Fluor 647 NBP2-44307AF647
Alexa Fluor 700 NBP2-44307AF700
Alexa Fluor 750 NBP2-44307AF750
Biotin NBP2-44307B
DyLight 350 NBP2-44307UV
DyLight 405 NBP2-44307V
DyLight 488 NBP2-44307G
DyLight 550 NBP2-44307R
DyLight 594 NBP2-44307DL594
DyLight 650 NBP2-44307C
DyLight 680 NBP2-44307FR
DyLight 755 NBP2-44307IR
FITC NBP2-44307F
HRP NBP2-44307H
Janelia Fluor 549 NBP2-44307JF549
Janelia Fluor 646 NBP2-44307JF646

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Blogs on CHREBP.

Deficiency of GluT1 leads to neurological problems while excess is involved in cancers
By Jamshed Arslan, Pharm. D., PhD. What Are GluTs?Mammalian cell metabolism is incomplete without glucose   . Glucose is a monosaccharide that is transported to the cells through facilitative diffusion, a...  Read full blog post.

Insulin signaling in adipocytes: Carbohydrate-signaling transcription factor ChREBP is the link between lipolytic enzyme Hormone-Sensitive Lipase and lipogenic enzyme ELOVL6
By Jamshed Arslan, Pharm. D., PhD. Insulin resistance in adipocytes is a major feature of metabolic syndrome   . Disrupted adipose tissue metabolism can lead to accumulation of lipid intermediates in insul...  Read full blog post.

ChREBP, a glucose sensitive transcription factor with role in glucose-lipids homeostasis and cancer
ChREBP (carbohydrate response element-binding protein) is a glucose responsive basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor that binds MLX and then carbohydrate response element /ChoRE for the induction of genes involved in...  Read full blog post.

ChREBP and Fatty Acid Biosynthesis Regulation
Carbohydrate response element binding protein (ChREBP) is a transcription factor involved in activating genes that encode enzymes of fatty acid biosynthesis in liver and adipose. It is believed to be a key controller of hepatic lipogenesis and has a p...  Read full blog post.

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Bioinformatics

Gene Symbol MLXIPL
Entrez
Uniprot