Chk1 Antibody

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Biological Strategies: Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody ...read more
Western Blot: Chk1 Antibody [NB100-311] - Whole Cell lysate (50 ug for WB, 500 ug for IP) from HeLa cells. Affinity purified goat anti-Chk1 used as indicated. Immunoprecipitated material was detected by WB using a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Chk1 Antibody Summary

Immunogen
The epitope recognized by NB100-311 maps to a region between residues 250 and 300 of human Checkpoint Kinase 1 using the numbering given in entry NP_001265.1 (GeneID 1111).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CHEK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 5-20 ug/mg lysate
  • Western Blot 1:1000-1:10000
Application Notes
100-311 may be used for Western Blot where a band at ~53 kD is seen on SDS-PAGE. It may also be used for immunoprecipitation (band ~ 48 kDa). Suggested working dilutions: * Western Blot 1:1,000-1:10,000 (ECL) Immunocytochemistry Not Done Immunoprecipitation 5-20 ug/g lysate *The investigator should determine the optimal working dilution for a specific application.
Control
Chk1 Overexpression Lysate
HeLa Whole Cell Lysate
Publications
Read Publications using NB100-311.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Chk1 Antibody

  • Checkpoint, S. pombe, homolog of, 1
  • CHEK1
  • CHK1 (checkpoint, S.pombe) homolog
  • CHK1 checkpoint homolog (S. pombe)
  • Chk1
  • CHK1serine/threonine-protein kinase Chk1
  • EC 2.7.11
  • EC 2.7.11.1

Background

In response to DNA damage and replication blocks, cells prevent cell cycle progression through the control of critical cell cycle regulators. Chk1 is an essential kinase for embryonic development and regulating mitotic progression in response to DNA damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Chk1 Antibody (NB100-311) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHEK1
Entrez