CHD8 Antibody

Western Blot: CHD8 Antibody [NB100-60418] - Detection of human and mouse CHD8 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, K-562, MCF-7, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-CHD8 antibody NB100-60418 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Immunohistochemistry-Paraffin: CHD8 Antibody [NB100-60418] - section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: CHD8 Antibody [NB100-60418] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: CHD8 Antibody [NB100-60418] - Detection of human CHD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CHD8 antibody NB100-60418 (lot NB100-60418-4) used for IP at 6 ul per reaction. CHD8 was also immunoprecipitated by a previous lot of this antibody (lot NB100-60418-3). For blotting immunoprecipitated CHD8, NB100-60418 was used at 0.1 ug/ml. Chemiluminescence with an exposure time of 1 second.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CHD8 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2252 and 2302 of human chromodomain helicase DNA binding protein 8 (NP_065971.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHD8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200- 1:1000
  • Immunohistochemistry-Paraffin 1:200- 1:1000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 290 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CHD8 Antibody

• ATP-dependent helicase CHD8
• CHD-8
• chromodomain helicase DNA binding protein 8
• DKFZp686N17164
• duplin
• EC 3.6.1
• EC 3.6.1.7
• EC 3.6.4.12
• Helicase with SNF2 domain 1chromodomain-helicase-DNA-binding protein 8
• HELSNF1
• KIAA1564axis duplication inhibitor

Background

CHD8 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD8 has been reported to interact with duplin, a protein involved in the wnt signaling pathway, and CTCF, a protein involved in heterochromatin formation. Alternate names for CHD8 include chromodomain-helicase-DNA-binding protein 8, ATP-dependent helicase CHD8, helicase with SNF2 domain 1, HELSNF1, and KIAA1564.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHD8 Antibody (NB100-60418)(6)

Publications using NB100-60418

• Kerschbamer E, Tripathi T, Erdin S, Salviato E CHD8 Suppression Impacts on Histone H3 Lysine 36 Trimethylation and Alters RNA Alternative Splicing. bioRxiv 2020-03-16 [PMID: 36537238]
• Villa CE, Cheroni C, Dotter CP et al. CHD8 haploinsufficiency links autism to transient alterations in excitatory and inhibitory trajectories Cell reports 2022-04-05 [PMID: 35385734] (WB, Human)
• Sood S, Weber CM, Hodges HC et al. CHD8 dosage regulates transcription in pluripotency and early murine neural differentiation Proc. Natl. Acad. Sci. U.S.A. 2020-08-24 [PMID: 32839322] (ICC/IF, KO, WB, Mouse)
• Damaschke NA, Yang B, Blute ML et al. Frequent Disruption of Chromodomain Helicase DNA-Binding Protein 8 (CHD8) and Functionally Associated Chromatin Regulators in Prostate Cancer. Neoplasia. 2014-12-01 [PMID: 25499215] (IHC-P, Human)
• Sugathan A, Biagioli M, Golzio C et al. CHD8 regulates neurodevelopmental pathways associated with autism spectrum disorder in neural progenitors. Proc Natl Acad Sci U S A 2014-10-21 [PMID: 25294932] (IP)
• Rodenberg JM, Hoggatt AM, Chen M et al. REGULATION OF SERUM RESPONSE FACTOR ACTIVITY AND SMOOTH MUSCLE CELL APOPTOSIS BY CHROMODOMAIN HELICASE DNA-BINDING PROTEIN 8. Am J Physiol Cell Physiol 2010-08-25 [PMID: 20739623]

FAQs for CHD8 Antibody (NB100-60418). (Showing 1 - 1 of 1 FAQs).

1. I see that all of your CHD8 antibodies say that they are specific for human. Have any been tested in mouse? I want to know if they have and were found not to be suitable. Ideally, I would like to try one in mouse for immunocytochemistry.
   • We have not tested any of our CHD8 antibodies in mouse samples, and do not have any data to support whether they will work for you or not. If you would like to purchase one to try on your samples, you will qualify for our Innovators Reward Program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data.

Bioinformatics

• Gene Symbol: CHD8
• Entrez:
  • 57680(Human)
• Uniprot:
  • Q9HCK8(Human)