CHD8 Antibody

Images

 
Western Blot: CHD8 Antibody [NB100-60418] - Detection of human and mouse CHD8 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, K-562, MCF-7, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: ...read more
Immunohistochemistry-Paraffin: CHD8 Antibody [NB100-60418] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Independent Antibodies: Western Blot: CHD8 Antibody [NB100-60418] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-60418 used for WB at 0.04 ug/ml (A) and 1 ...read more
Biological Strategies: Western Blot: CHD8 Antibody [NB100-60418] - Detection of Human and Mouse CHD8 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse ...read more
Independent Antibodies: Immunoprecipitation: CHD8 Antibody [NB100-60418] - Detection of human CHD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

CHD8 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 2252 and 2302 of human chromodomain helicase DNA binding protein 8 (NP_065971.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHD8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
290 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-60418 in the following applications:

  • 1 publication
  • IP
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CHD8 Antibody

  • ATP-dependent helicase CHD8
  • CHD-8
  • chromodomain helicase DNA binding protein 8
  • DKFZp686N17164
  • duplin
  • EC 3.6.1
  • EC 3.6.1.7
  • EC 3.6.4.12
  • Helicase with SNF2 domain 1chromodomain-helicase-DNA-binding protein 8
  • HELSNF1
  • KIAA1564axis duplication inhibitor

Background

CHD8 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD8 has been reported to interact with duplin, a protein involved in the wnt signaling pathway, and CTCF, a protein involved in heterochromatin formation. Alternate names for CHD8 include chromodomain-helicase-DNA-binding protein 8, ATP-dependent helicase CHD8, helicase with SNF2 domain 1, HELSNF1, and KIAA1564.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CHD8 Antibody (NB100-60418)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CHD8 Antibody (NB100-60418). (Showing 1 - 1 of 1 FAQs).

  1. I see that all of your CHD8 antibodies say that they are specific for human. Have any been tested in mouse? I want to know if they have and were found not to be suitable. Ideally, I would like to try one in mouse for immunocytochemistry.
    • We have not tested any of our CHD8 antibodies in mouse samples, and do not have any data to support whether they will work for you or not. If you would like to purchase one to try on your samples, you will qualify for our Innovators Reward Program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data.

Secondary Antibodies

 

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Array NB100-60418

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Bioinformatics

Gene Symbol CHD8
Entrez
Uniprot