Western Blot: CHD8 Antibody [NB100-60418] - Detection of human and mouse CHD8 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, K-562, MCF-7, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: ...read more
Immunohistochemistry-Paraffin: CHD8 Antibody [NB100-60418] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Independent Antibodies: Western Blot: CHD8 Antibody [NB100-60418] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-60418 used for WB at 0.04 ug/ml (A) and 1 ...read more
Biological Strategies: Western Blot: CHD8 Antibody [NB100-60418] - Detection of Human and Mouse CHD8 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse ...read more
Independent Antibodies: Immunoprecipitation: CHD8 Antibody [NB100-60418] - Detection of human CHD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from ...read more
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
290 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB100-60418 in the following applications:
Helicase with SNF2 domain 1chromodomain-helicase-DNA-binding protein 8
KIAA1564axis duplication inhibitor
CHD8 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD8 has been reported to interact with duplin, a protein involved in the wnt signaling pathway, and CTCF, a protein involved in heterochromatin formation. Alternate names for CHD8 include chromodomain-helicase-DNA-binding protein 8, ATP-dependent helicase CHD8, helicase with SNF2 domain 1, HELSNF1, and KIAA1564.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for CHD8 Antibody (NB100-60418). (Showing 1 - 1 of 1 FAQs).
I see that all of your CHD8 antibodies say that they are specific for human. Have any been tested in mouse? I want to know if they have and were found not to be suitable. Ideally, I would like to try one in mouse for immunocytochemistry.
We have not tested any of our CHD8 antibodies in mouse samples, and do not have any data to support whether they will work for you or not. If you would like to purchase one to try on your samples, you will qualify for our Innovators Reward Program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.