CARD10 Antibody

Images

 
Western Blot: CARD10 Antibody [NBP1-33042] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with CARD10 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunohistochemistry-Paraffin: CARD10 Antibody [NBP1-33042] - Paraffin-embedded human breast carcinoma. CARD10 antibody [N2C1], Internal diluted at 1:250.
Western Blot: CARD10 Antibody [NBP1-33042] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CARD10 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CARD10. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CARD10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 21078624).
Theoretical MW
116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-33042 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for CARD10 Antibody

  • CARD-containing MAGUK 3 protein
  • CARD-containing MAGUK protein 3
  • Carma 3
  • CARMA3BIMP1Bcl10 binding protein and activator of NFKB
  • caspase recruitment domain family, member 10
  • caspase recruitment domain-containing protein 10
  • MGC142219

Background

The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. CARDs induce nuclear factor kappa-B (NFKB; MIM 164011) activity through the IKK (e.g., IKBKB; MIM 603258) complex. CARD9 (MIM 607212), CARD10, CARD11 (MIM 607210), and CARD14 (MIM 607211) interact with BCL10 (MIM 603517) and are involved in NFKB signaling complexes. Except for CARD9, these CARD proteins are members of the membrane-associated guanylate kinase (MAGUK) family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CARD10 Antibody (NBP1-33042)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for CARD10 Antibody (NBP1-33042)

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Blogs on CARD10.

Toll-like receptor 2 activation contributes to oral squamous cell carcinoma development and miRNA-mediated drug resistance
By Jamshed Arslan, Pharm. D., PhD. Squamous cell carcinoma is the most common cancer in the oral cavity.1 The tumor surface biofilms in oral cancers contain high levels of aerobic and anaerobic microorganisms.1,2 Peri...  Read full blog post.

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Bioinformatics

Gene Symbol CARD10