CHD8 Antibody - BSA Free

Western Blot: CHD8 Antibody [NB100-60417] - Detection of Human and Mouse CHD8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CHD8 antibody NB100-60417 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: CHD8 Antibody [NB100-60417] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: CHD8 Antibody [NB100-60417] - Detection of human CHD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD8 antibody NB100-60417 used for IP at 6 ug/mg lysate. CHD8 was also immunoprecipitated by rabbit anti-CHD8 antibody NB100-60418. For blotting immunoprecipitated CHD8, NB100-60417 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CHD8 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit CHD8 Antibody - BSA Free (NB100-60417) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, IP and ChIP. Anti-CHD8 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 325 and 350 of human chromodomain helicase DNA binding protein 8 (NP_065971.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHD8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:33859315) Use in chromatin immunoprecipitation reported in scientific literature (PMID 25294932). Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 290 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CHD8 Antibody - BSA Free

• ATP-dependent helicase CHD8
• CHD-8
• chromodomain helicase DNA binding protein 8
• DKFZp686N17164
• duplin
• EC 3.6.1
• EC 3.6.1.7
• EC 3.6.4.12
• Helicase with SNF2 domain 1chromodomain-helicase-DNA-binding protein 8
• HELSNF1
• KIAA1564axis duplication inhibitor

Background

CHD8 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD8 has been reported to interact with duplin, a protein involved in the wnt signaling pathway, and CTCF, a protein involved in heterochromatin formation. Alternate names for CHD8 include chromodomain-helicase-DNA-binding protein 8, ATP-dependent helicase CHD8, helicase with SNF2 domain 1, HELSNF1, and KIAA1564.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHD8 Antibody (NB100-60417)(7)

Publications using NB100-60417

• Wade AA, Lim K, Catta-Preta R, Nord AS. Common CHD8 Genomic Targets Contrast With Model-Specific Transcriptional Impacts of CHD8 Haploinsufficiency. Front Mol Neurosci 2018-01-14 [PMID: 30692911] (Chemotaxis, Mouse)
• Platt RJ, Zhou Y, Slaymaker IM et al. Chd8 Mutation Leads to Autistic-like Behaviors and Impaired Striatal Circuits. Cell Rep. 2017-04-11 [PMID: 28402856] (Chemotaxis, Mouse)
• Sugathan A, Biagioli M, Golzio C et al. CHD8 regulates neurodevelopmental pathways associated with autism spectrum disorder in neural progenitors. Proc Natl Acad Sci U S A 2014-10-21 [PMID: 25294932] (Chemotaxis, Human)
• Wade A, Lim K, Catta-Preta R et al. CHD8 Regulates Cellular Homeostasis and Neuronal Function Genes Across Multiple Models ofCHD8Haploinsufficiency bioRxiv 2018-07-24 (Chemotaxis)
• Kerschbamer E, Tripathi T, Erdin S, Salviato E CHD8 Suppression Impacts on Histone H3 Lysine 36 Trimethylation and Alters RNA Alternative Splicing. bioRxiv 2020-03-16 [PMID: 36537238] (WB, Human)
• Cerase A, Young AN, Ruiz NB et al. Chd8 regulates X chromosome inactivation in mouse through fine-tuning control of Xist expression Communications biology 2021-04-15 [PMID: 33859315] (Chemotaxis, ICC/IF, WB)
• Sood S, Weber CM, Hodges HC et al. CHD8 dosage regulates transcription in pluripotency and early murine neural differentiation Proc. Natl. Acad. Sci. U.S.A. 2020-08-24 [PMID: 32839322] (KO, ICC/IF, WB, Mouse)

FAQs for CHD8 Antibody (NB100-60417). (Showing 1 - 1 of 1 FAQs).

1. I see that all of your CHD8 antibodies say that they are specific for human. Have any been tested in mouse? I want to know if they have and were found not to be suitable. Ideally, I would like to try one in mouse for immunocytochemistry.
   • We have not tested any of our CHD8 antibodies in mouse samples, and do not have any data to support whether they will work for you or not. If you would like to purchase one to try on your samples, you will qualify for our Innovators Reward Program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data.

Bioinformatics

• Gene Symbol: CHD8
• Entrez:
  • 57680(Human)
• Uniprot:
  • Q9HCK8(Human)