CHD8 Antibody

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Biological Strategies: Western Blot: CHD8 Antibody [NB100-60417] - Detection of Human and Mouse CHD8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. ...read more
Immunohistochemistry: CHD8 Antibody [NB100-60417] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Biological Strategies: Western Blot: CHD8 Antibody [NB100-60417] - Detection of Mouse CHD8 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity ...read more
Independent Antibodies: Western Blot: CHD8 Antibody [NB100-60417] - Detection of Human CHD8 on HeLa whole cell lysate (5, 15, and 50 microgram) using NB100-60417. CHD8 was also immunoprecipitated by rabbit ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CHD8 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 325 and 350 of human chromodomain helicase DNA binding protein 8 (NP_065971.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHD8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2 - 10 ug/mg lysate
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID 25294932)
Theoretical MW
290 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-60417 in the following applications:

  • 2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CHD8 Antibody

  • ATP-dependent helicase CHD8
  • CHD-8
  • chromodomain helicase DNA binding protein 8
  • DKFZp686N17164
  • DUPLIN
  • EC 3.6.1
  • EC 3.6.1.7
  • EC 3.6.4.12
  • Helicase with SNF2 domain 1chromodomain-helicase-DNA-binding protein 8
  • HELSNF1
  • KIAA1564axis duplication inhibitor

Background

CHD8 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD8 has been reported to interact with duplin, a protein involved in the wnt signaling pathway, and CTCF, a protein involved in heterochromatin formation. Alternate names for CHD8 include chromodomain-helicase-DNA-binding protein 8, ATP-dependent helicase CHD8, helicase with SNF2 domain 1, HELSNF1, and KIAA1564.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CHD8 Antibody (NB100-60417)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: ChIP.


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ChIP
(2)
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(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol

FAQs for CHD8 Antibody (NB100-60417). (Showing 1 - 1 of 1 FAQs).

  1. I see that all of your CHD8 antibodies say that they are specific for human. Have any been tested in mouse? I want to know if they have and were found not to be suitable. Ideally, I would like to try one in mouse for immunocytochemistry.
    • We have not tested any of our CHD8 antibodies in mouse samples, and do not have any data to support whether they will work for you or not. If you would like to purchase one to try on your samples, you will qualify for our Innovators Reward Program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data.

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Bioinformatics

Gene Symbol CHD8
Entrez
Uniprot