CHD7 Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human CHD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7350) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CHD7 at approximately 350 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

CHD7 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human CHD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7350) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CHD7 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CHD7
  Ala263-Gln457
  Accession # Q9P2D1
• Specificity: Detects human CHD7 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CHD7
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CHD7 Antibody [Unconjugated]

• ATP-dependent helicase CHD7
• CHD7
• CHD-7
• chromodomain helicase DNA binding protein 7 isoform CRA_e
• chromodomain helicase DNA binding protein 7
• chromodomain-helicase-DNA-binding protein 7
• EC 3.6.1
• EC 3.6.4.12
• FLJ20357
• FLJ20361
• IS3
• KIAA1416KAL5

Background

CHD7 (Chromohelicase/ATPase DNA-binding protein 7) is a 350 kDa member of the third subgroup in the SNF2/RAD54 helicase family of proteins. It is an ATP‑dependent chromatin remodeling factor that also binds histones (H3 at Lys4) and influences transcription. CHD7 binds more that 10,000 sites on chromatin, particularly in locations associated with gene enhancement. Notably, CHD7 is most associated with the nervous system, and is found in embryonic hypothalamus (GnRH neurons), olfactory epithelium and spinal cord, and adult preoptic hypothalamus plus hippocampus. Human CHD7 is 2997 amino acids (aa) in length. It contains consecutive Gln-, Pro- and Lys-rich regions (aa 151-718), two Chromo (chromatin-organizer-modifer) domains (aa 800-947), a helicase ATP-binding domain (aa 980-1154), a C-terminal helicase domain (aa 1294-1464), one coiled-coil region (aa 2410-2431) and two BRK domains (aa 2562-2604 and 2642-2686). There are at least seven utilized Ser/Thr phosphorylation sites, and three potential isoform variants. One isoform contains a 12 aa substitution for aa 1127-2997, a second 145 kDa isoform shows a deletion of aa 573-2621, while a third isoform contains a 15 aa insertion after Ser698. CHD7 is reported to bind to subgroup three member CHD8. Over aa 263-457, human CHD7 shares 96% aa identity with mouse CHD7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHD7 Antibody (AF7350)(1)

Publication using AF7350

• J Nie, Y Ueda, AJ Solivais, E Hashino CHD7 regulates otic lineage specification and hair cell differentiation in human inner ear organoids Nature Communications, 2022-11-17;13(1):7053. 2022-11-17 [PMID: 36396635] (IHC, Western Blot, Human)

Bioinformatics

• Gene Symbol: CHD7
• Uniprot:
  • Q9P2D1(Human)