GnRHR Antibody (HL2638)

GnRHR antibody [HL2638] detects GnRHR protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.GnRHR stained by GnRHR antibody [HL2638] (NBP3-48712) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Boiled and unboiled PC-3 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GnRHR antibody [HL2638] (NBP3-48712) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody

GnRHR antibody [HL2638] detects GnRHR protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human PC-3 xenograft.GnRHR stained by GnRHR antibody [HL2638] (NBP3-48712) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GnRHR antibody [HL2638] detects GnRHR protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded PC-3 xenograft.GnRHR stained by GnRHR antibody [HL2638] (NBP3-48712) diluted at 1:200 and competitor's antibody (19950-1-AP) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min*Competitor's antibody is not affiliated with and does not endorse this product.

Non-transfected (-) and transfected (+) unboiled PC-3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GnRHR antibody [HL2638] (NBP3-48712) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GnRHR antibody [HL2638] (NBP3-48712) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: HL2638
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

GnRHR Antibody (HL2638) Summary

• Description: Novus Biologicals Rabbit GnRHR Antibody (HL2638) (NBP3-48712) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide encompassing a sequence within the Extracellular domain of human GnRHR. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GNRHR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for GnRHR Antibody (HL2638)

• GnRH receptor
• GnRHR
• GnRH-R
• GNRHR1
• gonadotropin-releasing hormone (type 1) receptor 1
• gonadotropin-releasing hormone receptor
• GRHR
• HH7
• leutinizing-releasing hormone receptor
• LHRHR
• LHRHRleutinizing hormone releasing horomone receptor
• LRHR
• luliberin receptor
• Luliberin
• type I GnRH receptor

Background

GnRHR encodes the receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. More than 18 transcription initiation sites in the 5' region and multiple polyA signals in the 3' region have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GNRHR