CHAF1A Antibody (JG38-98)

Western Blot: CHAF1A Antibody (JG38-98) [NBP2-76859] - Western blot analysis of p150 CAF1 on K562 (1) and A431 (2) cell lysate using anti-p150 CAF1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (JG38-98) [NBP2-76859] - ICC staining p150 CAF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CHAF1A Antibody (JG38-98) [NBP2-76859] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-p150 CHAF1A antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: CHAF1A Antibody (JG38-98) [NBP2-76859] - Flow cytometric analysis of Daudi cells with p150 CAF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (JG38-98) [NBP2-76859] - ICC staining p150 CAF1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (JG38-98) [NBP2-76859] - ICC staining p150 CAF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: CHAF1A Antibody (JG38-98) [NBP2-76859] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p150 CAF1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: CHAF1A Antibody (JG38-98) [NBP2-76859] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p150 CAF1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CHAF1A Antibody (JG38-98) [NBP2-76859] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-CHAF1A antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: CHAF1A Antibody (JG38-98) [NBP2-76859] - Analysis of paraffin-embedded human testis tissue with Rabbit anti-CHAF1A antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

CHAF1A Antibody (JG38-98) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human CHAF1A aa 81-195 / 956. (SwissProt: Q13111 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CHAF1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 107 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CHAF1A Antibody (JG38-98)

• CAF
• CAF-1 subunit A
• CAF1
• CAF-1
• CAF1B
• CAF1P150CAF-I 150 kDa subunit
• CAF-I p150
• chromatin assembly factor 1 subunit A
• chromatin assembly factor 1, subunit A (p150)
• chromatin assembly factor I (150 kDa)
• Chromatin assembly factor I p150 subunit
• hp150
• MGC71229
• P150

Background

Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

CAF-1 antibodies are useful tools for cell division, DNA replication and DNA packaging.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHAF1A