CEACAM8/CD66b Antibody [Biotin]

Human whole blood granulocytes were stained with Biotinylated Sheep Anti-Human CEACAM‑8/CD66b Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF4246, filled histogram) or control antibody (Catalog # BAF020, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).

• Reactivity: Hu
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

CEACAM8/CD66b Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human CEACAM‑8/CD66b
  Gln35-His141
  Accession # P31997
• Specificity: Detects human CEACAM‑8/CD66b in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) CEACAM-3 and rhCEACAM-7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CEACAM8
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEACAM8/CD66b Antibody [Biotin]

• Carcinoembryonic antigen CGM6
• carcinoembryonic antigen gene family member 6
• carcinoembryonic antigen-related cell adhesion molecule 8
• CD66b antigen
• CD66b
• CD67 antigen
• CD67
• CEACAM8
• CEACAM-8
• CGM6
• NCA-95
• Non-specific cross-reacting antigen NCA-95

Background

CEACAM-8 (carcinoembryonic antigen-related cell adhesion molecule 8; also CD66b, CD67 and NCA-95) is a 90 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and eosinophils, and serves as a binding partner for CEACAM-6 and Galectin-3. Mature human CEACAM-8 is a 287 amino acid GPI-linked glycoprotein. It contains one V-type and two C2-type Ig-like domains. No definitive rodent CEACAM-8 has been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEACAM8/CD66b Antibody (BAF4246)(2)

Publications using BAF4246

• Hakan Inan, James L. Kingsley, Mehmet O. Ozen, Huseyin Cumhur Tekin, Christian R. Hoerner, Yoriko Imae et al. Monitoring Neutropenia for Cancer Patients at the Point of Care Small Methods 2017-09-11 [PMID: 30740513]
• Hakan Inan, James L. Kingsley, Mehmet O. Ozen, Huseyin Cumhur Tekin, Christian R. Hoerner, Yoriko Imae et al. Monitoring Neutropenia for Cancer Patients at the Point of Care Small Methods 2017-09-11 [PMID: 30740513] (Cell Capture, Human)

Bioinformatics

• Gene Symbol: CEACAM8
• Uniprot:
  • P31997()