CEACAM6/CD66c Antibody (1H7-4B) - BSA Free

Immunohistochemistry: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Analysis in human lung: Using Anti-CEACAM6 [1H7-4B] (10 ug/ml) developed with DAB-staining procedure. Dark line indicated shows CEACAM6 expression.

Flow Cytometry: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM6 [1H7-4B] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).

ELISA: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Solid phase was coated with 3 ug/ml Anti-CEA binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM6 [1H7-4B] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.

Immunoprecipitation: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Human granulocyte lysate: Utilizing 10 ug/ml Anti-CEACAM6 [1H7-4B]. Detection of the sample using 5 ug/ml pAb Anti-CEA followed by HRP-coupled secondary pAb. Visualized by Chemiluminescence detection.

Western Blot: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Detection using 10 μg/ml NBP2-54626 followed by HRP-coupled secondary antibody. Visualized by Chemiluminescence detection.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, IHC, CyTOF-ready
• Clone: 1H7-4B
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CEACAM6/CD66c Antibody (1H7-4B) - BSA Free Summary

• Description: Novus Biologicals Mouse CEACAM6/CD66c Antibody (1H7-4B) - BSA Free (NBP2-54626) is a monoclonal antibody validated for use in IHC, WB, ELISA and Flow. Anti-CEACAM6/CD66c Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human CEACAM6/CD66c extracellular domain.
• Specificity: Clone 1H7-4B does not cross-react with human CEACAM1/3/4/5/7/8, rat CECAM1, or mouse CEACAM1/2.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEACAM6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1 - 1000
  • Immunohistochemistry 1:100 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: This antibody is CyTOF ready.
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32031201).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CEACAM6/CD66c Antibody (1H7-4B) - BSA Free

• carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific crossreacting antigen)
• carcinoembryonic antigen-related cell adhesion molecule 6
• CD66c antigen
• CD66c
• CEACAM6
• CEACAM-6
• CEAL
• NCA
• NCACEAL
• Non-specific crossreacting antigen
• Normal cross-reacting antigen

Background

CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. CEACAM 6 expression is strongly up-regulated already during early stages of adenocarcinoma formation e.g. in colon. The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEACAM6/CD66c Antibody (NBP2-54626)(1)

Publication using NBP2-54626

• Zou J, Chen S, Li Y et al. Nanoparticles modified by triple single chain antibodies for MRI examination and targeted therapy in pancreatic cancer Nanoscale 2020-02-07 [PMID: 32031201] (Mouse)

Bioinformatics

• Gene Symbol: CEACAM6